人肿瘤细胞的药敏试验：对异种移植瘤的体外和体内试验
Assays of drug sensitivity for cells from human tumours: in vitro and in vivo tests on a xenografted tumour.
作者信息
Bateman A E, Peckham M J, Steel G G
出版信息
Br J Cancer. 1979 Jul;40(1):81-8. doi: 10.1038/bjc.1979.143.
Abstract
A human tumour which grows as a xenograft in immune-suppressed mice and forms colonies in vitro has been used to test the correlation between 2 methods of exposure of human tumour cells to chemotherapeutic agents. In vivo exposure to drugs was achieved by injection of tumour-bearing mice with each of 8 cytotoxic agents. For the in vitro exposure, cell suspensions were incubated for 1 h with the same series of drugs. The survival of tumour clonogenic cells was assayed in vitro after either treatment or dose-response curves were obtained. The 8 drugs were ranked according to their in vivo effect at doses equitoxic to mice, and according to their in vitro effect at concentrations designed to approximate to levels of drugs in human plasma. The ranks for in vivo and in vitro exposure correlated well.
摘要
一种能在免疫抑制小鼠体内以异种移植方式生长并在体外形成集落的人类肿瘤，已被用于测试人类肿瘤细胞接触化疗药物的两种方法之间的相关性。通过给荷瘤小鼠注射8种细胞毒性药物中的每一种来实现体内药物暴露。对于体外暴露，将细胞悬液与同一系列药物孵育1小时。在处理后或获得剂量反应曲线后，在体外测定肿瘤克隆形成细胞的存活率。根据8种药物对小鼠的等效毒性剂量下的体内效应，以及根据其在旨在接近人血浆中药物水平的浓度下的体外效应进行排名。体内和体外暴露的排名相关性良好。
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