菜豆细胞质、叶绿体和线粒体中的酶对酵母苯丙氨酸tRNA的甲基化作用。
Methylation of yeast tRNAPhe by enzymes from cytoplasm, chloroplasts and mitochondria of Phaseolus vulgaris.
作者信息
Montasser Kouhsari S, Keith G, Weil J H
出版信息
Biochim Biophys Acta. 1978 Dec 21;521(2):576-83. doi: 10.1016/0005-2787(78)90299-x.
Pure yeast tRNAPhe was used as a substrate to compare the tRNA methylating activities in Phaseolus vulgaris cytoplasm, chloroplasts and mitochondria, in the presence of S-adenosyl[Me-3H]methionine. The resulting [Me-3H]-tRNAPhe was then analyzed, using the techniques of nucleotide sequence determination. Cytoplasmic and mitochondrial enzymes catalyze the methylation (into m5C) of C48 present in the extra-loop, while chloroplast enzyme preparations catalyze the modification (into m1A) of A14 present in the dihydrouridine loop of tRNAPhe.
在存在S-腺苷[甲基-³H]甲硫氨酸的情况下，使用纯酵母苯丙氨酸tRNA作为底物，比较菜豆细胞质、叶绿体和线粒体中的tRNA甲基化活性。然后使用核苷酸序列测定技术对所得的[甲基-³H] - 苯丙氨酸tRNA进行分析。细胞质和线粒体酶催化额外环中存在的C48甲基化（形成m⁵C），而叶绿体酶制剂催化苯丙氨酸tRNA二氢尿嘧啶环中存在的A14修饰（形成m¹A）。
Zotero 插件