Itzkowitch D, Brauman H, Gregoire F, Staroukine M, Abramov M
Horm Metab Res. 1980 Mar;12(3):111-6. doi: 10.1055/s-2007-996216.
A radioimmunoassay of ADH has been applied to the study of plasma ADH levels in various conditions. The validity of the assay has been evaluated by the usual quality control parameters of RIA and by the measure of plasma levels in 12 upright water deprived normal volunteers (mean 9.5 pg/ml, SEM +/- 1.5) in 8 resting and hydrated normal volunteers (1.3 +/- 0.4 pg/ml), in a case of diabetes insipidus (1.6 pg/ml), in 8 cases of SIADH Syndrome (range 13-77 pg/ml) and in 4 anesthetized dogs before (33.7 +/- 9.2 pg/ml) and after acute haemorrhage (66 +/- 9.5 pg/ml, p less than 0.02). The osmotoic challenge to ADH secretion has been studied in 8 patients with no overt endocrine pathology by salt perfusion and showed a significant rise (p less than 0.05) of plasma ADH from 6.3 +/- 3.1 pg/ml before, to 20.6 +/- 7.9 pg/ml during salt infusion corresponding to the significant (p less than 0.0001) rise of plasma osmolality from 273 +/- 2.8 to 288.2 +/- 1.1 m Osm/kg.
抗利尿激素的放射免疫测定已应用于各种情况下血浆抗利尿激素水平的研究。该测定的有效性已通过放射免疫分析的常规质量控制参数以及对12名直立性禁水正常志愿者（平均9.5 pg/ml，标准误±1.5）、8名静息且水分充足的正常志愿者（1.3±0.4 pg/ml）、1例尿崩症患者（1.6 pg/ml）、8例抗利尿激素分泌异常综合征患者（范围13 - 77 pg/ml）以及4只麻醉犬在急性出血前后（出血前33.7±9.2 pg/ml，出血后66±9.5 pg/ml，p＜0.02）的血浆水平测量进行了评估。通过盐灌注对8名无明显内分泌病变的患者进行了抗利尿激素分泌的渗透压刺激研究，结果显示血浆抗利尿激素从灌注前的6.3±3.1 pg/ml显著升高（p＜0.05）至灌注期间的20.6±7.9 pg/ml，这与血浆渗透压从273±2.8显著升高（p＜0.0001）至288.2±1.1 mOsm/kg相对应。
