Winkler G C, Brown G M, Lutz H
J Clin Microbiol. 1987 Apr;25(4):633-6. doi: 10.1128/jcm.25.4.633-636.1987.
Sensitivities of two enzyme-linked immunosorbent assays (ELISAs) with particulate and sodium dodecyl sulfate (SDS)-disrupted Anaplasma marginale antigen were compared. The quotient of positive reference sera divided by the absorbance quotient of a negative reference serum at identical dilution was termed the signal-to-noise ratio. Optimal signal-to-noise ratios were dependent on both pretreatment of antigen and antigen concentration. SDS disruption of anaplasmal antigen resulted in a markedly improved signal-to-noise ratio of ELISA compared with ELISA with untreated antigen at identical antigen and serum dilutions. This represented higher sensitivity and lower background absorbance of the ELISA with disrupted antigen. SDS-disrupted A. marginale antigen was standardized by protein determination, and antigen, as well as precoated microtiter wells, was stored frozen without apparent loss of antigenic properties. ELISA results were in agreement with results of positive and negative control sera tested by the complement fixation test or by light microscopy Anaplasma diagnosis in Giemsa-stained blood films.
比较了两种酶联免疫吸附测定（ELISA）对颗粒状和经十二烷基硫酸钠（SDS）处理破坏的边缘无形体抗原的敏感性。将阳性参考血清的吸光度与相同稀释度下阴性参考血清的吸光度之商称为信噪比。最佳信噪比取决于抗原的预处理和抗原浓度。与在相同抗原和血清稀释度下使用未处理抗原的ELISA相比，SDS破坏无形体抗原后，ELISA的信噪比显著提高。这表明使用破坏抗原的ELISA具有更高的灵敏度和更低的背景吸光度。通过蛋白质测定对经SDS处理破坏的边缘无形体抗原进行标准化，抗原以及预包被的微量滴定板冷冻保存，抗原特性无明显损失。ELISA结果与通过补体结合试验或吉姆萨染色血片光学显微镜无形体诊断检测的阳性和阴性对照血清结果一致。
