Trider D J, Castell J D
J Nutr. 1980 Jul;110(7):1303-9. doi: 10.1093/jn/110.7.1303.
Hatchery reared oysters (Crassostrea virginica) were fed six different diets for 30 weeks. The diets contained cod liver oil (CLO), corn oil (CO), corn oil and cod liver oil (1:2) (CO + CLO), hyrogenated coconut oil (HCO), ethyl esters of cod liver oil fatty acids (SF) or cod liver oil ethyl esters supplemented with cholesterol (CH). Data on growth and tissue composition imply that oysters have an essential fatty acid (EFA) requirement for both linolenic or omega 3 and linoleic or omega 6 series fatty acids, with the former playing a more significant role. The feeding study supported by a 14C-acetate metabolism experiment suggests that these oysters were unable to synthesize sterols and excessively high dietary sterol (1% of the dry weight of the diet) inhibited growth.
将孵化场养殖的牡蛎（弗吉尼亚牡蛎）投喂六种不同的饲料，为期30周。这些饲料含有鳕鱼肝油（CLO）、玉米油（CO）、玉米油和鳕鱼肝油（1:2）（CO + CLO）、氢化椰子油（HCO）、鳕鱼肝油脂肪酸乙酯（SF）或添加胆固醇的鳕鱼肝油乙酯（CH）。生长和组织成分数据表明，牡蛎对亚麻酸或ω-3以及亚油酸或ω-6系列脂肪酸都有必需脂肪酸（EFA）需求，前者发挥着更重要的作用。一项由¹⁴C-乙酸代谢实验支持的投喂研究表明，这些牡蛎无法合成固醇，过高的日粮固醇（占日粮干重的1%）会抑制生长。
