Chapkin R S, Ziboh V A, McCullough J L
J Nutr. 1987 Aug;117(8):1360-70. doi: 10.1093/jn/117.8.1360.
There have been reports that certain dietary lipids are capable of regulating cellular inflammation and hyperproliferation. To investigate further the role of dietary manipulation involving gamma-linolenic acid (18:3n-6) and eicosapentaenoic acid (20:5n-3) on hyperproliferative cellular components, the effects of orally administered primrose oil (containing 18:3n-6) and menhaden fish oil (containing 20:5n-3) were tested in a cutaneous system using the essential fatty acid (EFA)-deficient guinea pig fed a hydrogenated coconut oil (HCO) diet. The effects of the dietary crossover regimen were determined on epidermal 1) morphology, 2) DNA synthesis, 3) delta 6- and delta 5-desaturase activities and 4) fatty acid composition of skin and liver lipids. Our results demonstrated that dietary fish oil lacked the capacity to reverse the signs of epidermal hyperproliferation, acanthosis and hypergranulosis that are characteristic of EFA deficiency. In contrast, primrose oil feeding reversed the histological and biochemical signs of hyperproliferation. These results suggest that dietary fish oil, which contains largely the 20:5n-3 fatty acid, lacks EFA-functional properties in the skin. In addition, substitution of HCO with primrose or fish oil after 6 wk revealed incorporation of 18:3n-6 and 20:5n-3 into epidermal lipids, respectively. The significance of these altered epidermal fatty acid profiles is discussed.
有报道称，某些膳食脂质能够调节细胞炎症和过度增殖。为了进一步研究涉及γ-亚麻酸（18:3n-6）和二十碳五烯酸（20:5n-3）的膳食调控对过度增殖细胞成分的作用，在一个使用缺乏必需脂肪酸（EFA）且喂食氢化椰子油（HCO）饮食的豚鼠的皮肤系统中，测试了口服月见草油（含18:3n-6）和鲱鱼油（含20:5n-3）的效果。测定了膳食交叉方案对表皮的以下方面的影响：1）形态学，2）DNA合成，3）δ6-和δ5-去饱和酶活性，以及4）皮肤和肝脏脂质的脂肪酸组成。我们的结果表明，膳食鱼油缺乏逆转EFA缺乏所特有的表皮过度增殖、棘皮症和颗粒层增厚迹象的能力。相比之下，喂食月见草油可逆转过度增殖的组织学和生化迹象。这些结果表明，主要含有20:5n-3脂肪酸的膳食鱼油在皮肤中缺乏EFA功能特性。此外，6周后用月见草油或鱼油替代HCO分别显示18:3n-6和20:5n-3掺入表皮脂质中。本文讨论了这些改变的表皮脂肪酸谱的意义。
