Johnston H H, Mitchell C J, Curtis G D
Lancet. 1976 Aug 21;2(7982):400-2. doi: 10.1016/s0140-6736(76)92411-9.
A fully automated technique for the rapid detection of significant bacteriuria is described. The method, which depends on the detection of bacterial adenosine triphosphate (A.T.P.) by bioluminescence, was found to have better sensitivity than a conventional cultural technique which uses a standardised loop. Results were available in 30 minutes. In the 957 urine samples tested, specificity was 84.5% relative to a pour-plate technique; results were not affected by the presence of non-bacterial elements.
本文描述了一种用于快速检测显著菌尿的全自动技术。该方法依赖于通过生物发光检测细菌三磷酸腺苷（ATP），结果发现其灵敏度优于使用标准化接种环的传统培养技术。30分钟即可获得结果。在957份受试尿液样本中，相对于倾注平板技术，其特异性为84.5%；结果不受非细菌成分的影响。
