Martinez A O, Norwood T H, Prothero J W, Martin G M
In Vitro. 1978 Dec;14(12):996-1002. doi: 10.1007/BF02616213.
The growth of primary clones and serial subclones of HeLa cells and of diploid human fibroblast-like cells were compared both in the presence and absence of feeder layers; the latter had no significant effects upon the results. Clones and subclones of both cell types displayed great heterogeneity in growth rates, typically with a bimodality of growth distributions. Serial passages of clones selected on the basis of superior rates of proliferation showed attentuation of growth potentials; the extent of such attentuations was much less in the case of HeLa cells, suggesting at least one possible basis for the differences in long-term growth potential between these two classes of cell lines.
在有饲养层和无饲养层的情况下，对HeLa细胞以及二倍体人成纤维细胞样细胞的原代克隆和连续亚克隆的生长进行了比较；饲养层对结果没有显著影响。两种细胞类型的克隆和亚克隆在生长速率上表现出很大的异质性，典型的是生长分布呈双峰性。基于较高增殖率选择的克隆进行连续传代显示出生长潜能的减弱；在HeLa细胞中这种减弱的程度要小得多，这表明这两类细胞系在长期生长潜能上存在差异的至少一个可能原因。
