Koop H, Lankisch P G, Stöckmann F, Arnold R
Digestion. 1980;20(3):151-6. doi: 10.1159/000198434.
Serum immunoreactive trypsin (IRT) determination has been recommended as a screening test in chronic pancreatitis. Using a commercial radioimmunoassay kit (RIA--gnost Trypsin; Behring-Werke, Marburg/Lahn, FRG) the interassay coefficient of variation was 26--44% for three different test sera. Gel filtration chromatography profiles revealed immunoreactivity in the position of 125I-trypsin and (less than 50%) in the void volume. The test was evaluated in controls (n = 90), chronic relapsing pancreatitis (CRP;n = 60) and after total pancreatectomy (n = 5). In 65% of the CRP cases decreased IRT values were found, whereas during acute attacks of CRP supranormal and normal values were found. After total pancreatectomy IRT levels were undetectable. It is concluded that the sensitivity of this IRT test is limited and that the available test system needs improvement.
血清免疫反应性胰蛋白酶（IRT）测定已被推荐作为慢性胰腺炎的筛查试验。使用一种商用放射免疫分析试剂盒（RIA - gnost Trypsin；德国马尔堡/拉恩的贝林werke公司），三种不同测试血清的批间变异系数为26 - 44%。凝胶过滤色谱图谱显示在125I - 胰蛋白酶位置有免疫反应性，在空体积处有（小于50%）免疫反应性。该试验在对照组（n = 90）、慢性复发性胰腺炎（CRP；n = 60）和全胰切除术后（n = 5）进行了评估。在65%的CRP病例中发现IRT值降低，而在CRP急性发作期间发现超正常和正常的值。全胰切除术后IRT水平检测不到。结论是该IRT试验的敏感性有限，现有的检测系统需要改进。
