Nishioka K, Katayama I
J Pathol. 1978 Oct;126(2):63-9. doi: 10.1002/path.1711260202.
The culture supernatant of antigen- and phytohaemagglutinin-stimulated lymph node cells contained angiogenic activity when applied to hamster cheek pouch vascular membranes. New capillary growth was observed about 7 days after such application. This activity was generated by culturing lymph node cells with antigens for at least 12 hr, while migration inhibitory factor (MIF) was detected in the culture supernatant of 6 hr cultures. The angiogenic activity was separated from blastogenic activity, clot-promoting activity and antibody activity. However, it was not completely separated from MIF by gel-filtration. The molecular weight of the angiogenic activity was estimated to be between 35,000 and 55,000 daltons.
当将抗原和植物血凝素刺激的淋巴结细胞的培养上清液应用于仓鼠颊囊血管膜时，其具有血管生成活性。在应用后约7天观察到新的毛细血管生长。这种活性是通过将淋巴结细胞与抗原培养至少12小时产生的，而在6小时培养的上清液中检测到迁移抑制因子（MIF）。血管生成活性与增殖活性、促凝活性和抗体活性分离。然而，通过凝胶过滤它并未与MIF完全分离。血管生成活性的分子量估计在35,000至55,000道尔顿之间。
