Karthik Yalpi, Kalyani Manjula Ishwara, Krishnappa Srinivasa, Ramakrishna Krishnaveni, Sayed Samy M, Aharthy Ohud Muslat, Alhelaify Seham Sater, Mushtaq Muntazir
Department of Studies and Research in Microbiology, Mangalore University, Kodagu, Karnataka, India.
Department of Studies and Research in Biochemistry, Mangalore University, Kodagu, Karnataka, India.
Front Microbiol. 2023 Jun 16;14:1152985. doi: 10.3389/fmicb.2023.1152985. eCollection 2023.
is a group of microbes known for antibiotic production and has contributed to more than 70% of present commercially available antibiotics. These antibiotics are important in the management, protection, and treatment of chronic illnesses. In the present study, the isolated strain from mangrove soil in Mangalore, India (GenBank accession number: MW785875) was subjected for differential cultural characterization, phenotype involving brown pigmentation, filamentous mycelia, and ash-colored spore production was observed using field emission scanning electron microscopy (FESEM) analysis revealing filamentous mycelia possessing a straight spore chain. Spores were visualized as elongated, rod-shaped, smooth surfaces with curved edges. After optimized growth conditions for on starch-casein agar medium, the GC/MS analysis of intracellular extract detected bioactive compounds reported for pharmacological applications. Analyzed using the NIST library, most of the bioactive compounds identified in intracellular extract had molecular weights of less than 1 kDa. On the PC3 cell line, the Sephadex G-10 partially purified eluted peak protein fraction demonstrated significant anticancer activity. The LCMS analysis revealed the presence of Tryprostatin B, Fumonisin B1, Microcystin LR, and Surfactin C with molecular weights below 1 kDa. This study found that small molecular weight microbial compounds are more effective in a variety of biological applications.
是一组以产生抗生素而闻名的微生物，目前市面上超过70%的抗生素都来源于此。这些抗生素在慢性病的管理、防护和治疗中具有重要作用。在本研究中，从印度芒格洛尔的红树林土壤中分离出的菌株（GenBank登录号：MW785875）进行了差异培养特征分析，通过场发射扫描电子显微镜（FESEM）分析观察到其表型包括棕色色素沉着、丝状菌丝体和灰白色孢子产生，显示丝状菌丝体具有直的孢子链。孢子呈细长的杆状，表面光滑，边缘弯曲。在淀粉 - 酪蛋白琼脂培养基上优化生长条件后，对细胞内提取物进行GC/MS分析，检测到有药理应用报道的生物活性化合物。使用NIST库进行分析，细胞内提取物中鉴定出的大多数生物活性化合物分子量小于1 kDa。在PC3细胞系上，Sephadex G - 10部分纯化的洗脱峰蛋白组分显示出显著的抗癌活性。LCMS分析显示存在分子量低于1 kDa的曲古抑菌素B、伏马毒素B1、微囊藻毒素LR和表面活性素C。本研究发现小分子微生物化合物在多种生物学应用中更有效。
