van de Donk H J, Zuidema J, Merkus F W
Rhinology. 1980 Jun;18(2):93-104.
A method for measurement of tracheal ciliary beat frequency in vitro is described. Light transmitted through the cilia is detected by a phototransistor mounted in a microscope, while the frequency is measured instantaneously and the waveform is displayed by an oscilloscope, connected to a transient recorder. Due to the magnification and the method of illumination, the movement of approximately 30 cilia is projected on the phototransistor. In Locke-Ringer solution the waveform shows a very constant amplitude. Interference arises after a noxious influence and is dependent on the frequency of the ciliary movement. The effect of pH and osmotic pressure on chicken embryo and rat tracheal ciliary beat frequency is assessed. The frequency is not influenced between pH = 7 and pH = 10, but higher and lower pH values decrease the frequency. Hypertonic NaCl solutions decrease the frequency of chicken embryo cilia as much as hypotonic NaCl solutions. Rat cilia turned out to be less sensitive for hypotonic NaCl solutions.
本文描述了一种体外测量气管纤毛搏动频率的方法。安装在显微镜中的光电晶体管检测透过纤毛的光，频率由瞬态记录仪连接的示波器即时测量并显示波形。由于放大倍数和照明方法，约30根纤毛的运动投影在光电晶体管上。在洛克林格溶液中，波形显示出非常恒定的幅度。有害影响后会出现干扰，且干扰取决于纤毛运动的频率。评估了pH值和渗透压对鸡胚和大鼠气管纤毛搏动频率的影响。pH值在7至10之间时频率不受影响，但更高和更低的pH值会降低频率。高渗氯化钠溶液与低渗氯化钠溶液一样会降低鸡胚纤毛的频率。结果表明大鼠纤毛对低渗氯化钠溶液的敏感性较低。
