Schummer U, Schiefer H G
Biochim Biophys Acta. 1980 Aug 14;600(3):993-7. doi: 10.1016/0005-2736(80)90501-5.
A new method for the determination of electrical potentials across cellular membranes has been developed. In order to determine the membrane potential, cells were incubated in buffer solutions with increasing concentrations of KCl. Parallel experiments were performed with buffer solutions which additonally contained valinomycin. After sedimentation of the cells, the membrane potential was calculated from data which were obtained by simply measuring the wet mass, the dry mass and the potassium content of cell pellets by atomic absorption spectroscopy.
一种测定细胞膜跨膜电位的新方法已被开发出来。为了测定膜电位，将细胞置于氯化钾浓度不断增加的缓冲溶液中进行孵育。同时，使用额外含有缬氨霉素的缓冲溶液进行平行实验。细胞沉降后，通过原子吸收光谱法简单测量细胞沉淀的湿质量、干质量和钾含量所获得的数据来计算膜电位。
