Ito A, Yamagiwa H, Sasaki R
J Am Geriatr Soc. 1980 Sep;28(9):398-404. doi: 10.1111/j.1532-5415.1980.tb01106.x.
Hydroxyproline concentration in the NaCl-soluble, TCA-soluble, and residual fractions of heart muscle was chemically determined in 40 autopsy subjects (25 men and 15 women), to study the effects of aging. In the left ventricle, the total hydroxyproline content of the endocardium and papillary muscle increased significantly in relation to age; the increase was greatest in the TCA-soluble fraction, followed by the residual fraction. Statistical analysis revealed that hydroxyproline in the residual fraction increased from the epicardium to the endocardium and then to the papillary muscle. Significant negative correlations were found between heart weight and total hydroxyproline in the epicardium and endocardium. In the right ventricle, a significant negative correlation was noted between heart weight and total hydroxyproline content. Though no correlations were evident between age and hydroxyproline content in females, significant correlations were observed between age and the total hydroxyproline content of the endocardium and papillary muscle in males. Furthermore, soluble collagen increased in most parts of the heart in females, but insoluble collagen increased in all parts of the heart in males. It is concluded that the hydroxyproline content of heart muscle increases in proportion to age and is in inverse proportion to heart weight. The increase occurs in the TCA-soluble and residual fractions, and extends from the endocardium to the papillary muscle. More profound changes in collagen metabolism are observed in males.
为研究衰老的影响，对40例尸检对象（25名男性和15名女性）的心肌中氯化钠可溶性、三氯乙酸可溶性及残余部分的羟脯氨酸浓度进行了化学测定。在左心室，心内膜和乳头肌的总羟脯氨酸含量随年龄显著增加；三氯乙酸可溶性部分增加最大，其次是残余部分。统计分析显示，残余部分的羟脯氨酸从心外膜到心内膜再到乳头肌逐渐增加。在心外膜和心内膜中，心脏重量与总羟脯氨酸之间存在显著负相关。在右心室，心脏重量与总羟脯氨酸含量之间存在显著负相关。虽然女性的年龄与羟脯氨酸含量之间无明显相关性，但男性的年龄与心内膜和乳头肌的总羟脯氨酸含量之间存在显著相关性。此外，女性心脏大部分区域的可溶性胶原蛋白增加，而男性心脏所有区域的不可溶性胶原蛋白增加。结论是，心肌的羟脯氨酸含量随年龄成比例增加，与心脏重量成反比。这种增加发生在三氯乙酸可溶性和残余部分，且从心内膜延伸至乳头肌。男性的胶原蛋白代谢变化更为显著。
