Brown P B, Lewis K O
Ann Clin Biochem. 1980 Jul;17(4):192-8. doi: 10.1177/000456328001700407.
A method for serum alkaline phosphatase isoenzymes using an enzyme reaction rate analyser is described. The complete urea-induced degradation of enzyme activity is monitored, from which individual isoenzyme activities are obtained by calculating the constituent exponential components of the degradation curve. Activities have been measured with adequate sensitivity and selectivity for up to four isoenzyme components in normal and in pathological sera. The identity of each isoenzyme present is assigned from its characteristic degradation half-life, and by this method bone and liver alkaline phosphatase are clearly distinguished and quantitated, and a composite value for placental-intestinal alkaline phosphatase activity is obtained. The approach promises to be applicable to a wide range of isoenzymes, and in analogy with 'reaction rate' the term 'reaction rate retardation' is suggested for the procedure.
本文描述了一种使用酶反应速率分析仪检测血清碱性磷酸酶同工酶的方法。监测尿素诱导的酶活性完全降解过程，通过计算降解曲线的组成指数成分来获得各个同工酶的活性。该方法对正常和病理血清中多达四种同工酶成分的检测具有足够的灵敏度和选择性。根据每种同工酶的特征降解半衰期确定其身份，通过这种方法可以清晰地区分和定量骨碱性磷酸酶和肝碱性磷酸酶，并获得胎盘-肠碱性磷酸酶活性的综合值。该方法有望适用于多种同工酶，类似于“反应速率”，建议将该过程称为“反应速率延迟”。
