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组织培养中的人类大脑。I. 脑细胞培养物的获取、初步处理及建立

Human brain in tissue culture. I. Acquisition, initial processing, and establishment of brain cell cultures.

作者信息

Gilden D H, Devlin M, Wroblewska Z, Friedman H, Rorke L B, Santoli D, Koprowski H

出版信息

J Comp Neurol. 1975 Jun 1;161(3):295-306. doi: 10.1002/cne.901610302.

DOI:10.1002/cne.901610302
PMID:239028
Abstract

This paper details the in vitro techniques used to establish cells in culture from the brains of 40 patients, most of whom had chronic neurologic disease. The clinical and pathologic features of these patients are given. The success in establihsing cell lines was dependent upon the origin of tissue (biopsy vs. autopsy), the site of removal from the brain, and various environmental and technical manipulations in vitro.

摘要

本文详细介绍了用于从40例患者大脑中建立培养细胞的体外技术，其中大多数患者患有慢性神经系统疾病。文中给出了这些患者的临床和病理特征。建立细胞系的成功与否取决于组织来源（活检与尸检）、大脑中取材的部位以及体外的各种环境和技术操作。

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