Volden G, Thorsrud A K, Bjørnson I, Jellum E
J Invest Dermatol. 1980 Nov;75(5):421-4. doi: 10.1111/1523-1747.ep12524077.
The biochemical composition of blister fluid was compared with serum and with blister fluid from erythematous lesions induced by ultraviolet irradiation. By using glass capillary gas chromatography--mass spectrometry over 100 metabolites were determined and with the aid of two-dimensional high resolution electrophoresis (the ISO-DALT system) several hundred protein spots were seen. The results show that the suction blister fluid qualitatively have a serum-like pattern but that the concentration of each compound was smaller than in serum. Also in suction blisters raised on erythematous reactions induced by ultraviolet light the same pattern was seen. The content of sodium, potassium and chloride was the same in suction blisters raised on erythematous and normal skin as that of serum.
将水疱液的生化成分与血清以及紫外线照射诱发的红斑性皮损产生的水疱液进行了比较。通过玻璃毛细管气相色谱 - 质谱联用技术测定了100多种代谢物，并借助二维高分辨率电泳（ISO - DALT系统）观察到数百个蛋白质斑点。结果表明，抽吸水疱液在质量上具有类似血清的模式，但每种化合物的浓度均低于血清。在紫外线诱发的红斑反应上产生的抽吸水疱中也观察到相同的模式。在红斑皮肤和正常皮肤上产生的抽吸水疱中钠、钾和氯的含量与血清中的相同。
