Vermeer B J, Reman F C, van Gent C M
J Invest Dermatol. 1979 Oct;73(4):303-5. doi: 10.1111/1523-1747.ep12531833.
The concentration of 5 different proteins in suction blister fluid and serum was determined by immunotechniques. These proteins, varying in size and molecular weight (6,600-2,300,000) were insulin, albumin, high density lipoprotein determined as apoprotein A-I, alpha 2-macroglobulin and low density lipoprotein measured as apoprotein B. The difference in the blister fluid/serum concentration ratio of the proteins was dependent on the molecular weight and followed mainly the law of diffusion. Moreover, the amounts of insulin, albumin and apoproteins A-I and B in suction blister fluid were the same as those reported in peripheral lymph. The results indicate that the sieve function of the capillary basement membrane remains intact during the formation of the suction blisters. Suction blister fluid might therefore be regarded as representative of interstitial fluid. The concentrations of 4 different lipids (cholesterol, cholesterolesters, triglycerides and phospholipids) were also determined and their blister fluid/serum concentration ratio proved to have a fairly constant value of 0.25.
采用免疫技术测定了抽吸水疱液和血清中5种不同蛋白质的浓度。这些蛋白质大小和分子量各异（6600 - 2300000），分别为胰岛素、白蛋白、以载脂蛋白A - I测定的高密度脂蛋白、α2 - 巨球蛋白以及以载脂蛋白B测定的低密度脂蛋白。蛋白质的水疱液/血清浓度比差异取决于分子量，主要遵循扩散规律。此外，抽吸水疱液中胰岛素、白蛋白以及载脂蛋白A - I和B的含量与外周淋巴中报道的含量相同。结果表明，在抽吸水疱形成过程中，毛细血管基底膜的筛滤功能保持完整。因此，抽吸水疱液可被视为组织间液的代表。还测定了4种不同脂质（胆固醇、胆固醇酯、甘油三酯和磷脂）的浓度，其水疱液/血清浓度比被证明具有相当恒定的值0.25。
