吸疱液的脂质分析：细胞间液和血浆中脂质的比较。
Lipid profiling of suction blister fluid: comparison of lipids in interstitial fluid and plasma.
机构信息
Section for Ophthalmology, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Department of Clinical Neuroscience at Institute of Neuroscience and Physiology, Drottning Silvias Barn- och Ungdomssjukhus, Tillväxtcentrum, Vitaminvägen 21, 416 50, Göteborg, Sweden.
出版信息
Lipids Health Dis. 2019 Aug 24;18(1):164. doi: 10.1186/s12944-019-1107-3.
BACKGROUND
Recent technical advances in the extraction of dermal interstitial fluid (ISF) have stimulated interest in using this rather unexploited biofluid as an alternative to blood for detection and prediction of disease. However, knowledge about the presence of useful biomarkers for health monitoring in ISF is still limited. In this study, we characterized the lipidome of human suction blister fluid (SBF) as a surrogate for pure ISF and compared it to that of plasma.
METHODS
Plasma and SBF samples were obtained from 18 healthy human volunteers after an overnight fast. Total lipids were extracted and analyzed by liquid chromatography-tandem mass spectrometry. One hundred ninety-three lipid species covering 10 complex lipid classes were detected and quantified in both plasma and SBF using multiple reaction monitoring. A fraction of the lipid extract was subjected to alkaline transesterification and fatty acid methyl esters were analyzed by gas chromatography-mass spectrometry.
RESULTS
The total concentration of lipids in SBF was 17% of the plasma lipid concentration. The molar fraction of lipid species within lipid classes, as well as total fatty acids, showed a generally high correlation between plasma and SBF. However, SBF had larger fractions of lysophospholipids and diglycerides relative to plasma, and consequently less diacylphospholipids and triglycerides. Principal component analysis revealed that the interindividual variation in SBF lipid profiles was considerably larger than the within-subject variation between plasma and SBF.
CONCLUSIONS
Plasma and SBF lipid profiles show high correlation and SBF could be used interchangeably with blood for the analysis of major lipids used in health monitoring.
背景
最近在提取皮肤间质液（ISF）方面的技术进展激发了人们的兴趣，希望将这种尚未充分利用的生物流体用作替代血液，用于疾病的检测和预测。然而，关于 ISF 中存在用于健康监测的有用生物标志物的知识仍然有限。在这项研究中，我们对人体抽吸水疱液（SBF）的脂质组进行了表征，将其作为纯 ISF 的替代物，并将其与血浆进行了比较。
方法
在禁食过夜后，从 18 名健康的人类志愿者中获得血浆和 SBF 样本。通过液相色谱-串联质谱法提取并分析总脂质。使用多重反应监测，在血浆和 SBF 中检测并定量了 193 种脂质，涵盖了 10 种复杂脂质类别的脂质。对脂质提取物的一部分进行碱性酯交换，并用气相色谱-质谱法分析脂肪酸甲酯。
结果
SBF 中的总脂质浓度为血浆脂质浓度的 17%。脂质类别的脂质种类的摩尔分数以及总脂肪酸在血浆和 SBF 之间具有很高的相关性。然而，与血浆相比，SBF 中溶血磷脂和二酰甘油的比例较大，而二酰基磷脂和甘油三酯的比例较小。主成分分析表明，SBF 脂质谱的个体间变异性明显大于血浆和 SBF 之间的个体内变异性。
结论
血浆和 SBF 脂质谱具有高度相关性，并且 SBF 可以与血液互换用于健康监测中使用的主要脂质的分析。
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