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1
Purification of the lectin from Datura stramonium.从曼陀罗中纯化凝集素。
Biochem J. 1978 Dec 1;175(3):1151-3. doi: 10.1042/bj1751151.
2
Datura stramonium lectin.曼陀罗凝集素
Methods Enzymol. 1982;83:368-73. doi: 10.1016/0076-6879(82)83032-2.
3
Some properties of the lectin from Datura stramonium (thorn-apple) and the nature of its glycoprotein linkages.曼陀罗凝集素的一些特性及其糖蛋白连接的性质。
Biochem J. 1981 Aug 1;197(2):345-53. doi: 10.1042/bj1970345.
4
Tissue and subcellular distribution of the lectin from Datura stramonium (thorn apple).曼陀罗（洋金花）凝集素的组织和亚细胞分布。
Biochem J. 1979 Nov 15;184(2):215-9. doi: 10.1042/bj1840215.
5
Purification and some properties of a lectin from the fruit juice of the tomato (Lycopersicon esculentum).番茄（番茄属）果汁中一种凝集素的纯化及某些特性
Biochem J. 1980 Jan 1;185(1):269-72. doi: 10.1042/bj1850269.
6
Lectin affinity high-performance liquid chromatography: interactions of N-glycanase-released oligosaccharides with leukoagglutinating phytohemagglutinin, concanavalin A, Datura stramonium agglutinin, and Vicia villosa agglutinin.凝集素亲和高效液相色谱法：N-聚糖酶释放的寡糖与白细胞凝集植物血凝素、伴刀豆球蛋白A、曼陀罗凝集素和绒毛豌豆凝集素的相互作用
Anal Biochem. 1987 Nov 15;167(1):62-75. doi: 10.1016/0003-2697(87)90134-5.
7
Datura stramonium agglutinin released histamine from rat peritoneal mast cells that was inhibited by pertussis toxin, haptenic sugar and N-acetylglucosamine-specific lectins: involvement of glycoproteins with N-acetylglucosamine residues.曼陀罗凝集素可从大鼠腹膜肥大细胞中释放组胺，百日咳毒素、半抗原糖和N - 乙酰葡糖胺特异性凝集素可抑制这种释放：含N - 乙酰葡糖胺残基的糖蛋白参与其中。
Jpn J Pharmacol. 1994 Oct;66(2):195-204. doi: 10.1254/jjp.66.195.
8
Analysis of lectin properties with membrane ultrafiltration and high-pressure liquid chromatography.利用膜超滤和高压液相色谱法分析凝集素特性
J Biochem. 1992 May;111(5):623-6. doi: 10.1093/oxfordjournals.jbchem.a123808.
9
Datura lectin is both an anti-mitogen and a co-mitogen acting synergistically with phorbol ester.曼陀罗凝集素既是一种抗有丝分裂原，也是一种与佛波酯协同作用的共刺激原。
Scand J Immunol. 1988 Jan;27(1):31-4. doi: 10.1111/j.1365-3083.1988.tb02320.x.
10
Structural studies of the carbohydrate moieties of lectins from potato (Solanum tuberosum) tubers and thorn-apple (Datura stramonium) seeds.马铃薯（Solanum tuberosum）块茎和曼陀罗（Datura stramonium）种子中凝集素碳水化合物部分的结构研究。
Biochem J. 1982 Jan 1;201(1):199-208. doi: 10.1042/bj2010199.

引用本文的文献

1
Specificity of widely used lectins as probed with oligosaccharide and plant polysaccharide arrays.广泛使用的凝集素与寡糖和植物多糖阵列的特异性研究。
Histochem Cell Biol. 2024 Dec;162(6):495-510. doi: 10.1007/s00418-024-02323-8. Epub 2024 Aug 25.
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Sugar-Binding Profiles of Chitin-Binding Lectins from the Hevein Family: A Comprehensive Study.橡胶素家族几丁质结合凝集素的糖结合谱：一项综合研究
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Elicitor induction of the synthesis of a novel lectin-like arabinosylated hydroxyproline-rich glycoprotein in suspension cultures of Phaseolus vulgaris L.激发子诱导菜豆悬浮培养细胞合成一种新型的阿拉伯糖基化富含羟脯氨酸的凝集素样糖蛋白。
Planta. 1987 Oct;172(2):184-91. doi: 10.1007/BF00394586.
5
Differential erythrocyte agglutination pattern in normal and cancer patients with Synadenium grantii root (Hook f) lectin.正常人和癌症患者使用绿玉树根凝集素后的红细胞凝集差异模式
Indian J Clin Biochem. 2001 Jan;16(1):110-2. doi: 10.1007/BF02867578.
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Purification and Some Properties of a Daucus carota Lectin which Enhances the Activation of Prophenoloxidase by CaCl(2).一种能增强氯化钙对酚氧化酶原激活作用的胡萝卜凝集素的纯化及其某些性质
Plant Physiol. 1990 Jun;93(2):657-61. doi: 10.1104/pp.93.2.657.
7
Isolation and Partial Characterization of a New Lectin from Seeds of the Greater Celandine (Chelidonium majus).从白屈菜（Chelidonium majus）种子中分离并部分鉴定一种新凝集素
Plant Physiol. 1985 Jun;78(2):379-83. doi: 10.1104/pp.78.2.379.
8
Antinutritional factors in anasazi and other pinto beans (Phaseolus vulgaris L.).纳瓦霍豆及其他斑豆（菜豆属普通菜豆）中的抗营养因子。
Plant Foods Hum Nutr. 1997;51(2):85-98. doi: 10.1023/a:1007924931856.
9
A comparative study of lectin binding to cultured chick sternal chondrocytes and intact chick sternum.凝集素与培养的鸡胸骨软骨细胞及完整鸡胸骨结合的比较研究。
Glycoconj J. 1997 Apr;14(3):365-77. doi: 10.1023/a:1018526813159.
10
Isolation of a lectin from the pericarp of potato (Solanum tuberosum) fruits.从马铃薯（Solanum tuberosum）果实的果皮中分离出一种凝集素。
Biochem J. 1980 Oct 1;191(1):273-5. doi: 10.1042/bj1910273.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
THE HYDROLYSIS OF CHITIN BY CONCENTRATED HYDROCHLORIC ACID, AND THE PREPARATION OF LOW-MOLECULAR-WEIGHT SUBSTRATES FOR LYSOZYME.浓盐酸对几丁质的水解作用以及溶菌酶低分子量底物的制备
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Diffusion-in-gel methods for immunological analysis.用于免疫分析的凝胶扩散法。
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4
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
5
Lectins: cell-agglutinating and sugar-specific proteins.凝集素：细胞凝集性且具有糖特异性的蛋白质。
Science. 1972 Sep 15;177(4053):949-59. doi: 10.1126/science.177.4053.949.
6
The purification and properties of the lectin from potato tubers, a hydroxyproline-containing glycoprotein.来自马铃薯块茎的凝集素（一种含羟脯氨酸的糖蛋白）的纯化及性质
Biochem J. 1973 Oct;135(2):307-14. doi: 10.1042/bj1350307.
7
The interactions of lectins with animal cell surfaces.凝集素与动物细胞表面的相互作用。
Int Rev Cytol. 1974;39:89-190. doi: 10.1016/s0074-7696(08)60939-0.
8
Human N-acetyl-beta-hexosaminidases: hydrolysis of N, N' diacetylchitobiose by a low molecular weight enzyme.人N-乙酰-β-己糖胺酶：一种低分子量酶对N，N'-二乙酰壳二糖的水解作用。
FEBS Lett. 1974 Feb 15;39(2):171-5. doi: 10.1016/0014-5793(74)80044-x.
9
Glycoproteins of cell surfaces. A comparative study of three different cell surfaces of the rat.细胞表面糖蛋白。大鼠三种不同细胞表面的比较研究。
J Biol Chem. 1971 Oct 25;246(20):6339-46.
10
Isolation of lectins of different specificities on a single affinity adsorbent.在单一亲和吸附剂上分离不同特异性的凝集素。
J Biol Chem. 1975 Sep 25;250(18):7535-8.

从曼陀罗中纯化凝集素。

Purification of the lectin from Datura stramonium.

作者信息

Kilpatrick D C, Yeoman M M

出版信息

Biochem J. 1978 Dec 1;175(3):1151-3. doi: 10.1042/bj1751151.

DOI:10.1042/bj1751151
PMID:743232
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1186181/
Abstract

The lectin from Datura stramonium can be inhibited by oligomers of N-acetylglucosamine. This property was exploited to purify the lectin by affinity chromatography on Sepharosefetuin. The purified lectin is a glycoprotein in having subunits of 40 000 and 45 000 mol.wt.

摘要

曼陀罗凝集素可被N-乙酰葡糖胺的寡聚物抑制。利用这一特性，通过在琼脂糖胎球蛋白上进行亲和层析来纯化该凝集素。纯化后的凝集素是一种糖蛋白，其亚基的分子量分别为40000和45000。