稳定核心颗粒的分离与物理特性分析

de Murcia G, Mazen A, Erard M, Pouyet J, Champagne M

Nucleic Acids Res. 1980 Feb 25;8(4):767-79.

Core particles were prepared from mature chicken erythrocytes chromatin, according to the method of Lutter (J. Mol. Biol. 124, 391, 1978) with one major modification: after the second digestion, zonal centrifugation was used to isolate the core particle, instead of chromatography on Sepharose 6B. By using circular dichroism and electron microscopy, we were able to follow each step of the preparation and to offer an explanation of the discrepancies found in previous preparations and in our own preparations.

核心颗粒是根据Lutter的方法（《分子生物学杂志》124卷，391页，1978年），从成熟鸡红细胞染色质中制备的，有一个主要的修改：在第二次消化后，使用区带离心法分离核心颗粒，而不是在琼脂糖6B上进行色谱分离。通过使用圆二色性和电子显微镜，我们能够跟踪制备的每一步，并对先前制备以及我们自己的制备中发现的差异做出解释。