支原体DNA中的甲基化碱基。
Methylated bases in mycoplasmal DNA.
作者信息
Razin A, Razin S
出版信息
Nucleic Acids Res. 1980 Mar 25;8(6):1383-90. doi: 10.1093/nar/8.6.1383.
Abstract
The DNAs of four Mycoplasma and one Acholeplasma species were found to contain methylated bases. All of the five species contained 6-methyladenine (m6Ade), the methylated base characteristic of prokaryotic DNA. The extent of methylation of adenine residues in the mycoplasmal DNA ranged from 0.2% in Mycoplasma capricolum to about 2% in Mycoplasma arginini and Mycoplasma hyorhinis with intermediate methylation values for Mycoplasma orale and Acholeplasma laidlawii DNAs. About 5.8% of the cytosine residues in M. hyorhinis DNA were methylated also. Analysis of cell culture DNA for the presence of m6Ade as a means for detection of contamination by mycoplasmas, and the phylogenetic implications of the finding of methylated bases in mycoplasmal DNAs are discussed.
摘要
发现四种支原体和一种无胆甾原体的DNA含有甲基化碱基。这五个物种均含有6-甲基腺嘌呤（m6Ade），这是原核生物DNA的甲基化碱基特征。支原体DNA中腺嘌呤残基的甲基化程度范围从山羊支原体中的0.2%到精氨酸支原体和鼻支原体中的约2%，口腔支原体和莱氏无胆甾原体DNA的甲基化值处于中间水平。鼻支原体DNA中约5.8%的胞嘧啶残基也被甲基化。本文讨论了分析细胞培养DNA中m6Ade的存在作为检测支原体污染的一种方法，以及支原体DNA中甲基化碱基发现的系统发育意义。
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