Umlas J, O'Neill T P
Transfusion. 1980 Nov-Dec;20(6):720-4. doi: 10.1046/j.1537-2995.1980.20681057164.x.
A common method for measuring deglycerolization of previously frozen erythrocytes is to determine the osmolality of the supernatant fluid after completion of the wash cycle. We attempted to compare this type of examination with the simpler, faster, and cheaper determination of refractive index (RI) using the Goldberg refractometer. One hundred twenty units of deglycerolized red blood cells, some with glycerol added so as to exceed an acceptable 1% glycerol content, had measurements made of the post-wash supernatant fluid by refractive index and osmometry. The responsiveness of refractive index to residual glycerol was essentially the same as osmolality. Although there is more interference from supernatant hemoglobin, this did not preclude the use of RI to measure glycerol removal. Readings up to 28 had glycerol levels of less than 1%. Readings between 29 and 31 should be checked by osmometry, and readings over 31 should cause the unit of blood to be rejected.
测量先前冷冻红细胞脱甘油化程度的一种常用方法是在洗涤循环完成后测定上清液的渗透压。我们试图将这种检查方法与使用戈德堡折射仪进行的更简单、快速且成本更低的折射率（RI）测定进行比较。对120单位的脱甘油红细胞进行了检测，其中一些添加了甘油，使得甘油含量超过了可接受的1%。通过折射率和渗透压测定法对洗涤后的上清液进行了测量。折射率对残留甘油的反应性与渗透压基本相同。尽管上清液中的血红蛋白干扰更大，但这并不妨碍使用RI来测量甘油去除情况。读数高达28时，甘油水平低于1%。读数在29至31之间应通过渗透压测定法进行检查，读数超过31时应拒收该单位血液。
