Vizcaino O, Corrier D E, Terry M K, Carson C A, Lee A J, Kuttler K L, Ristic M, Treviño G S
Am J Vet Res. 1980 Jul;41(7):1066-8.
The efficacy of three immunization methods for bovine anaplasmosis was tested on 88 yearling Normandy cattle which were challenge-exposed under field conditions in an enzootic zone in Colombia, South American. A total of 30 cattle were immunized (premunized) with a presumed mild Colombian isolate of Anaplasma marginale; 29 calves were vaccinated with an attenuated A marginale of ovine origin; and 29 were exposed to a virulent A marginale stabilate of Colombian origin. Twenty-nine nonvaccinated cattle served as controls. Parasitemia, packed cell volume, body weight, and complement-fixation antibody response were monitored to determine host response to challenge exposure. The calves experienced a Babesia challenge exposure following arrival in the enzootic zone, after which a transient increase in Anaplasma parasitemia, accompanied by a decrease in packed cell volume, occurred in most of the vaccinated calves. However, neither clinical anaplasmosis nor mortality due to the disease occurred in the immunized cattle. Twenty-seven of the nonvaccinated calves had signs of clinical anaplasmosis and five (17%) died of the disease.
在南美洲哥伦比亚一个地方性流行区的野外条件下，对88头一岁龄诺曼底牛进行攻毒暴露试验，以测试三种牛无浆体病免疫方法的效果。总共30头牛用一种假定为温和型的哥伦比亚边缘无浆体分离株进行免疫（预先免疫）；29头犊牛用一种绵羊源减毒边缘无浆体进行疫苗接种；29头牛暴露于一种哥伦比亚源强毒边缘无浆体稳定株。29头未接种疫苗的牛作为对照。监测寄生虫血症、红细胞压积、体重和补体结合抗体反应，以确定宿主对攻毒暴露的反应。犊牛抵达地方性流行区后经历了巴贝斯虫攻毒暴露，之后大多数接种疫苗的犊牛出现边缘无浆体寄生虫血症短暂增加，同时红细胞压积下降。然而，免疫的牛既未发生临床无浆体病，也未出现因该病导致的死亡。27头未接种疫苗的犊牛出现临床无浆体病体征，5头（17%）死于该病。
