Cybulsky M I, McComb D J, Movat H Z
Department of Pathology, University of Toronto, Ontario, Canada.
Am J Pathol. 1989 Jul;135(1):227-37.
Inflammation constitutes the body's principal mode of defense against infection and other harmful agents. Neutrophil leukocytes are the primary effector cells in this process. The role of protein synthesis in neutrophil emigration into acute inflammatory lesions was examined. Local intradermal injections of actinomycin D, cycloheximide or puromycin could inhibit in a dose- and time-dependent manner neutrophil emigration induced by interleukin-1, tumor necrosis factor alpha or endotoxin, but not by the leukocyte chemoattractants C5a des arg (zymosan-activated plasma), n-formyl-methionyl-leucyl-phenylalanine or leukotriene B4. Maximal inhibition, measured at the time of peak emigration, was greater than 90%. The onset of neutrophil emigration induced by the cytokines or by endotoxin was delayed by 30 to 60 minutes in comparison to the leukocyte chemoattractants. These results demonstrate at least two mechanisms of neutrophil emigration: one with a slower onset and dependence on local RNA transcription and translation and the other rapid in onset and independent of protein synthesis.
炎症是机体抵御感染及其他有害物质的主要防御方式。中性粒细胞是这一过程中的主要效应细胞。研究了蛋白质合成在中性粒细胞迁移至急性炎症病灶中的作用。局部皮内注射放线菌素D、环己酰亚胺或嘌呤霉素，可剂量和时间依赖性地抑制白细胞介素-1、肿瘤坏死因子α或内毒素诱导的中性粒细胞迁移，但对白细胞趋化因子C5a des arg（酵母聚糖激活血浆）、N-甲酰甲硫氨酰亮氨酰苯丙氨酸或白三烯B4诱导的中性粒细胞迁移无抑制作用。在迁移高峰时测得的最大抑制率大于90%。与白细胞趋化因子相比，细胞因子或内毒素诱导的中性粒细胞迁移起始时间延迟30至60分钟。这些结果表明中性粒细胞迁移至少有两种机制：一种起始较慢，依赖局部RNA转录和翻译；另一种起始迅速，不依赖蛋白质合成。
