Brunnemann K D, Fink W, Moser F
Oncology. 1980;37(4):217-22. doi: 10.1159/000225439.
Volatile N-nitrosamines were quantitatively determined in mainstream and sidestream smoke of commercial cigarettes using gas chromatography-thermal energy analysis (GLC-TEA). The smoke was trapped in ascorbic acid solution buffered at pH 4.5 and then extracted with dichloromethane; the organic phase was chromatographed on basic alumina and analyzed by GLC-TEA. The mainstream smoke of 22 different commercial cigarettes from Germany and Switzerland contained between 0.1 and 27 ng dimethylnitrosamine (DMN), 1.5 and 29 ng nitrosopyrrolidine (NPY) and traces of methylethylnitrosamine (MEN). The sidestream smoke of these cigarettes contained between 143 and 415 ng DMN, 3.1 and 27 ng MEN and 28 and 150 ng NPY. In a final experiment we measured the DMN concentration in a sealed experimental chamber as a function of the number of cigarettes smoked and recovered 68-87% of the DMN values found when using the sidestream collection device applied in the other experiments.
采用气相色谱 - 热能分析（GLC - TEA）对市售香烟主流烟气和侧流烟气中的挥发性N - 亚硝胺进行了定量测定。将烟气捕集在pH值为4.5的抗坏血酸缓冲溶液中，然后用二氯甲烷萃取；有机相在碱性氧化铝上进行色谱分析，并通过GLC - TEA进行分析。来自德国和瑞士的22种不同市售香烟的主流烟气中，含有0.1至27纳克二甲基亚硝胺（DMN）、1.5至29纳克亚硝基吡咯烷（NPY）以及痕量的甲基乙基亚硝胺（MEN）。这些香烟的侧流烟气中含有143至415纳克DMN、3.1至27纳克MEN以及28至150纳克NPY。在最后一项实验中，我们测量了密封实验室内DMN的浓度与吸烟支数的关系，得到的DMN值回收率为68 - 87%，与在其他实验中使用侧流收集装置时测得的值相当。
