Sahu S P, Dardiri A H
Am J Vet Res. 1980 Sep;41(9):1379-82.
Uterine, cervical, and clitoral specimens on swabs from pony mares infected with contagious equine equine metritis (CEM) bacteria were streaked on agar plates. Colonies of CEM bacteria were observed under CO2 incubation in 2 days on Eugon chocolate agar and Eugon blood agar plates. The diameter of the colonies varied from 0.2 mm to 1 mm in 2 days which increased to 0.3 mm to 2.0 mm on day 4. The colonies on Eugon chocolate agar plates on days 2 to 4 were shiny, brown, round, and convex, and easily glided when pushed with a loop. The diameter of the colonies on chocolate and blood agar plates made from tryptose blood agar base (TrCA or TrBA) was 0.2 to 0.3 mm on day 4. Due to their small size on TrCA or TrBA, colonies of CEM bacteria were easily recognized from large numbers of contaminants. The organism required hemin for its growth. It gelled in water, caused delayed hemolysis of blood agar plates, and was extremely susceptible to acid in the pH range to 3 to 4.5. A difference in growth of CEM bacterium was observed on primary isolation media obtained from two different commerical sources.
将感染马传染性子宫炎（CEM）细菌的母马子宫、宫颈和阴蒂拭子标本接种于琼脂平板上。在二氧化碳培养条件下，2天后在优贡巧克力琼脂平板和优贡血琼脂平板上观察到CEM细菌菌落。2天时菌落直径在0.2毫米至1毫米之间，第4天增至0.3毫米至2.0毫米。第2至4天，优贡巧克力琼脂平板上的菌落有光泽、呈褐色、圆形且凸起，用接种环推动时易于滑动。用胰蛋白胨血琼脂基础培养基（TrCA或TrBA）制成的巧克力琼脂平板和血琼脂平板上的菌落，在第4天时直径为0.2至 0.3毫米。由于其在TrCA或TrBA上的菌落体积较小，CEM细菌菌落很容易从大量污染物中识别出来。该微生物生长需要血红素。它在水中形成凝胶，导致血琼脂平板出现延迟溶血，并且在pH值为3至4.5的范围内对酸极为敏感。在从两种不同商业来源获得的初代分离培养基上观察到CEM细菌生长存在差异。
