Lennon A M, Francon J, Fellous A, Nunez J
J Neurochem. 1980 Oct;35(4):804-13. doi: 10.1111/j.1471-4159.1980.tb07076.x.
The development of in vitro microtubule assembly and of tubulin concentration have been studied during brain maturation in the mouse and the rat, two species which have postnatal brain development, and in one species which is mature at birth, the guinea pig. (a) The rat of tubulin assembly is very slow soon after birth in both the mouse and rat; it increases progressively with age until adulthood. In contrast, in the guinea pig this rate is maximal at birth and slower rates are seen only at foetal stages. (b) Postnatal changes in the lag period of assembly and in the minimal concentration of tubulin (Cc) required to obtain in vitro assembly are seen in the mouse and the rat; in contrast these parameters are constant at all postnatal stages in the guinea pig with longer lag periods and lower Cc values being seen only at foetal stages. (c) Maximal rates of assembly, minimal lag periods, and minimal Cc values are restored after addition of microtubule-associated proteins to foetal guinea pig or young mouse and rat preparations, suggesting that the difference in the kinetic parameters of assembly between these species depends on differences in the concentration or activity of these proteins. (d) Maximal tubulin concentrations are observed before birth in the guinea pig and approximately at day 10 in the rat and mouse.
在小鼠和大鼠（这两种动物出生后脑仍在发育）以及豚鼠（一种出生时即成熟的动物）的脑成熟过程中，对体外微管组装和微管蛋白浓度的变化进行了研究。(a) 小鼠和大鼠出生后不久，微管蛋白组装速度都非常缓慢；随着年龄增长逐渐加快，直至成年。相比之下，豚鼠的这一速度在出生时最快，仅在胎儿期才出现较慢的速度。(b) 小鼠和大鼠出生后组装延迟期和体外组装所需微管蛋白的最低浓度（Cc）会发生变化；相比之下，豚鼠出生后所有阶段这些参数都是恒定的，只有在胎儿期才会出现较长的延迟期和较低的Cc值。(c) 向胎儿豚鼠或幼年小鼠和大鼠的制剂中添加微管相关蛋白后，组装的最大速度、最短延迟期和最低Cc值得以恢复，这表明这些物种之间组装动力学参数的差异取决于这些蛋白质的浓度或活性差异。(d) 豚鼠在出生前观察到微管蛋白浓度最高，大鼠和小鼠约在第10天达到最高。
