van den Berg W B, Haasakker T C, Bax J, Scheper R J
Immunology. 1980 Dec;41(4):981-8.
Studies were carried out in order to explain the often small contribution of eosinophils to immunologically mediated chronic inflammatory reactions. A chronic inflammation model was used in which large numbers of eosinophils accumulated in the peritoneal injections with PPD or ovalbumin (OA). Eosinophil accumulation could be strongly suppressed by pre-immunization with a mycobacteria-containing adjuvant, which induces delayed hypersensitivity to the stimulating antigen. Suppression of OA-induced eosinophil accumulation could be obtained in a non-specific way by concomitant delayed hypersensitivity reactions to PPD. The absence o eosinophil accumulation was not related to a lack of relevant antibodies. Histological studies suggested that similar numbers of basophils were locally available in both non-pre-immunized and pre-immunized groups to allow for the attraction of eosinophils by the expression of basophil anaphylactic reactivity. Skin tests and macrophage migration inhibition measurements provided further support for the hypothesis that local lymphokine release may suppress the accumulation of eosinophils in chronic inflammatory lesions.
开展了多项研究以解释嗜酸性粒细胞在免疫介导的慢性炎症反应中通常贡献较小的原因。使用了一种慢性炎症模型，在该模型中，在腹腔注射结核菌素纯蛋白衍生物（PPD）或卵白蛋白（OA）时会有大量嗜酸性粒细胞积聚。用含分枝杆菌的佐剂进行预免疫可强烈抑制嗜酸性粒细胞的积聚，该佐剂可诱导对刺激抗原的迟发型超敏反应。通过对PPD的伴随迟发型超敏反应，可通过非特异性方式抑制OA诱导的嗜酸性粒细胞积聚。嗜酸性粒细胞积聚的缺失与相关抗体的缺乏无关。组织学研究表明，在未预免疫组和预免疫组中，局部可得的嗜碱性粒细胞数量相似，以允许通过嗜碱性粒细胞过敏反应性的表达来吸引嗜酸性粒细胞。皮肤试验和巨噬细胞迁移抑制测量为局部淋巴因子释放可能抑制嗜酸性粒细胞在慢性炎症病变中积聚这一假说提供了进一步支持。
