Ristroph J D, Hedlund K W, Gowda S
J Clin Microbiol. 1981 Jan;13(1):115-9. doi: 10.1128/jcm.13.1.115-119.1981.
A chemically defined medium containing 18 amino acids, inorganic salts, rhamnose, choline, and ferric pyrophosphate has been developed. The final concentrations of salts and amino acids were modeled after yeast extract. This medium supported the growth of four serogroups of Legionella pneumophila. Growth in shake cultures at 37 degrees C produced a lag time of approximately 5 h and a generation time of 4 h with a maximum growth yield of 10 9 colony-forming units per ml. A soluble brown pigment was observed in the stationary phase of growth. The optimal pH was 6.3. Rhamnose and choline were stimulatory; arginine, serine, threonine, cysteine, valine, and methionine were essential. Supplemental iron was not required to attain maximum growth, but iron deprivation caused an extended lag phase.
一种含有18种氨基酸、无机盐、鼠李糖、胆碱和焦磷酸铁的化学成分明确的培养基已被研制出来。盐类和氨基酸的最终浓度是以酵母提取物为模型设定的。这种培养基支持嗜肺军团菌四个血清群的生长。在37℃摇瓶培养中，生长的延迟期约为5小时，代时为4小时，最大生长产量为每毫升10⁹个菌落形成单位。在生长的稳定期观察到一种可溶性棕色色素。最适pH值为6.3。鼠李糖和胆碱具有刺激作用；精氨酸、丝氨酸、苏氨酸、半胱氨酸、缬氨酸和蛋氨酸是必需的。达到最大生长并不需要额外添加铁，但缺铁会导致延迟期延长。
