Scott P M, Harwig J, Blanchfield B J
Mycopathologia. 1980 Nov 28;72(3):175-80. doi: 10.1007/BF00572661.
A survey of 38 samples of Canadian overwintered grains showed that 14 (37%) contained viable Fusarium. Of a total of 38 Fusarium isolates, cultured on autoclaved corn, 20 (from 7 grain samples) showed toxicity to brine shrimp larvae and 12 (from 5 samples) produced levels of trichothecenes detectable by thin layer chromatography. The principal trichothecene found was T-2 toxin, produced by 10 strains and accompanied in half of these by neosolaniol; some of these strains were identified as F. sporotrichioides Sherbakoff. Two strains of F. poae (Peck) Wollenw. formed small amounts of diacetoxyscirpenol. T-2 toxin was the most toxic of 8 trichothecenes tested on brine shrimp larvae; the wide range of toxicities limits the usefulness of this bioassay as a general screening method for trichothecenes.
一项对38份加拿大越冬谷物样本的调查显示，14份（37%）含有活的镰刀菌。在总共38株在高压灭菌玉米上培养的镰刀菌分离株中，20株（来自7个谷物样本）对卤虫幼虫表现出毒性，12株（来自5个样本）产生了可通过薄层色谱检测到的单端孢霉烯水平。发现的主要单端孢霉烯是T-2毒素，由10个菌株产生，其中一半伴有新茄病镰刀菌烯醇；其中一些菌株被鉴定为拟枝孢镰刀菌。两株梨孢镰刀菌产生少量的二乙酰氧基雪腐镰刀菌烯醇。T-2毒素是在卤虫幼虫上测试的8种单端孢霉烯中毒性最强的；毒性范围广泛限制了这种生物测定作为单端孢霉烯一般筛选方法的实用性。
