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筛查参与引发食源性中毒性白细胞缺乏症的禾谷镰刀菌和拟枝孢镰刀菌的毒性分离株。

Screeing of toxic isolates of Fusarium poae and Fusarium sporotrichiodes involved in causing alimentary toxic aleukia.

作者信息

Yagen B, Joffe A Z

出版信息

Appl Environ Microbiol. 1976 Sep;32(3):423-7. doi: 10.1128/aem.32.3.423-427.1976.

DOI:10.1128/aem.32.3.423-427.1976
PMID:984817
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC170081/
Abstract

A total of 131 isolates of Fusarium poae and F. sporotrichioides from overwintered cereals, which were associated with the alimentary toxic aleukia toxicoses in the Soviet Union, were tested for their ability to produce T-2 toxin [4 beta, 15 diacetoxy-8alpha-(3-methylbutyryloxy)-12,13-epoxytrichothec-9-en 3alpha-ol]. The presence of T-2 toxin was determined by thin-layer chromatography, gas-liquid chromatography, spectroscopic analyses, and the rabbit skin test. A good correlation was demonstrated between T-2 toxin dectetion by thin-layer chromatography and inflammatory skin reactions of rabbits.

摘要

对从越冬谷物中分离出的131株梨孢镰刀菌和拟枝孢镰刀菌进行了检测，这些菌株在苏联曾与 alimentary toxic aleukia中毒有关，检测它们产生T-2毒素[4β,15-二乙酰氧基-8α-(3-甲基丁酰氧基)-12,13-环氧单端孢霉烯-9-烯-3α-醇]的能力。通过薄层色谱法、气液色谱法、光谱分析和兔皮试验来测定T-2毒素的存在。薄层色谱法检测T-2毒素与兔的皮肤炎症反应之间显示出良好的相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b74/170081/e302c5ada0ba/aem00008-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b74/170081/e302c5ada0ba/aem00008-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b74/170081/e302c5ada0ba/aem00008-0128-a.jpg

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