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嗜热四膜虫分离核仁中核糖体RNA前体的体外剪接

In vitro splicing of the ribosomal RNA precursor in isolated nucleoli from Tetrahymena.

作者信息

Carin M, Jensen B F, Jentsch K D, Leer J C, Nielsen O F, Westergaard O

出版信息

Nucleic Acids Res. 1980 Dec 11;8(23):5551-66. doi: 10.1093/nar/8.23.5551.

DOI:10.1093/nar/8.23.5551
PMID:7465422
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324325/
Abstract

The macronuclear rRNA genes of Tetrahymena thermophila contain an 0.4 kb intervening sequence in the 26S rRNA coding region. The sequence is represented within the primary transcription product. We demonstrate in this paper that the enzyme activities necessary for the endonucleolytic cleavage as well as for the ligation of the transcript are associated with the isolated. The intervening sequence is excised as an unique molecule, which is stable in vitro. About 50% of the in vitro synthesized RNA is processed. Faithful in vitro transcription occurs in the presence of the divalent ions Mg2+, Mn2+ and Co2+ while processing takes place only in the presence of Mg2+. The absolute requirement for Mg2+ in the excision reaction enables us to synthesize labelled pre-rRNA in the presence of Mn2+ or Co2+. The synthesized RNA can be used as a substrate in studies of th processing enzymes in vitro.

摘要

嗜热四膜虫的大核rRNA基因在26S rRNA编码区含有一个0.4 kb的间隔序列。该序列存在于初级转录产物中。我们在本文中证明，内切核酸酶切割以及转录本连接所需的酶活性与分离物相关。间隔序列作为一个独特的分子被切除，它在体外是稳定的。约50%的体外合成RNA被加工。在二价离子Mg2+、Mn2+和Co2+存在的情况下发生忠实的体外转录，而加工仅在Mg2+存在时发生。切除反应对Mg2+的绝对需求使我们能够在Mn2+或Co2+存在的情况下合成标记的前体rRNA。合成的RNA可作为体外加工酶研究的底物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfad/324325/53067e8a6959/nar00440-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfad/324325/7fd333b36255/nar00440-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfad/324325/4c596d6de19c/nar00440-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfad/324325/867114cce66b/nar00440-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfad/324325/8d71b2078bc4/nar00440-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfad/324325/53067e8a6959/nar00440-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfad/324325/7fd333b36255/nar00440-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfad/324325/4c596d6de19c/nar00440-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfad/324325/867114cce66b/nar00440-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfad/324325/8d71b2078bc4/nar00440-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfad/324325/53067e8a6959/nar00440-0034-a.jpg

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In vitro splicing of the ribosomal RNA precursor in isolated nucleoli from Tetrahymena.嗜热四膜虫分离核仁中核糖体RNA前体的体外剪接
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In vitro splicing of the ribosomal RNA precursor of Tetrahymena: involvement of a guanosine nucleotide in the excision of the intervening sequence.嗜热四膜虫核糖体RNA前体的体外剪接：鸟苷酸在间隔序列切除中的作用。
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The intervening sequence in the 26S rRNA coding region of T. thermophila is transcribed within the largest stable precursor for rRNA.嗜热栖热菌26S rRNA编码区的间隔序列在rRNA最大的稳定前体中被转录。
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引用本文的文献

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2
The intervening sequence excised from the ribosomal RNA precursor of Tetrahymena contains a 5-terminal guanosine residue not encoded by the DNA.从四膜虫核糖体RNA前体切除的居间序列含有一个DNA未编码的5'-末端鸟苷残基。
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3
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本文引用的文献

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Structural considerations in the interaction of DNA and acridines.DNA与吖啶相互作用中的结构考量
J Mol Biol. 1961 Feb;3:18-30. doi: 10.1016/s0022-2836(61)80004-1.
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In vitro splicing of the ribosomal RNA precursor in nuclei of Tetrahymena.嗜热四膜虫细胞核内核糖体RNA前体的体外剪接
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Sequence of a ribosomal RNA gene intron from Tetrahymena.来自四膜虫的核糖体RNA基因内含子序列。
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DNase I hypersensitive regions correlate with a site-specific endogenous nuclease activity on the r-chromatin of Tetrahymena.脱氧核糖核酸酶I高敏区域与嗜热四膜虫r染色质上的位点特异性内源性核酸酶活性相关。
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Characterization of an authentic intermediate in the self-splicing process of ribosomal precursor RNA in macronuclei of Tetrahymena thermophila.嗜热栖热四膜虫大核核糖体前体RNA自我剪接过程中真实中间体的表征
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Evidence for the role of double-helical structures in the maturation of simian virus-40 messenger RNA.双链螺旋结构在猴病毒40信使核糖核酸成熟过程中作用的证据。
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