Lipson S J, Muir H
Arthritis Rheum. 1981 Jan;24(1):12-21. doi: 10.1002/art.1780240103.
An animal model of intervertebral disc degeneration produced by surgical ventral disc herniation in the rabbit is described. Histologic studies showed proliferation of cells on the inner third of the annular wound, with metaplasia into fibrocartilage in the first 2 weeks following injury. Progressive fibrocartilaginous change occurred, reproducing the morphologic changes of disc degeneration over the first 6 weeks involving nearly the entire disc. Proteoglycans (total and newly synthesized) were studied qualitatively and quantitatively for periods of 1 to 200 days after herniation. There were two periods of time during the early course of degeneration when the ability of the proteoglycans to aggregate by interaction with hyaluronic acid was recovered, but this decreases progressively after 6-7 weeks. There was an immediate loss of water content from the injured disc which was restored only transiently during the first 2 days after herniation. Thereafter the water content progressively decreased. The uronic acid content of the disc changed in parallel with the changes in water content. The hyaluronic acid content decreased rapidly after herniation. However, the size of the proteoglycan monomers did not change with degeneration. The biochemical and morphologic changes are correlated, and an early repair mechanism is postulated to exist after injury.
描述了一种通过兔手术性腹侧椎间盘突出产生的椎间盘退变动物模型。组织学研究显示，在环形伤口内三分之一处细胞增殖，损伤后的前2周内化生为纤维软骨。发生了进行性纤维软骨变化，在最初6周内重现了几乎累及整个椎间盘的退变形态学变化。在椎间盘突出后的1至200天内，对蛋白聚糖（总量和新合成量）进行了定性和定量研究。在退变早期有两个时间段，蛋白聚糖通过与透明质酸相互作用而聚集的能力得以恢复，但在6 - 7周后这种能力逐渐下降。受伤椎间盘的含水量立即减少，仅在椎间盘突出后的头2天短暂恢复。此后含水量逐渐降低。椎间盘的糖醛酸含量随含水量变化而平行变化。椎间盘突出后透明质酸含量迅速降低。然而，蛋白聚糖单体的大小并未随退变而改变。生化和形态学变化相关，推测损伤后存在早期修复机制。
