Mekinová D, Chorváthová V, Volkovová K, Staruchová M, Grancicová E, Klvanová J, Ondreicka R
Research Institute of Nutrition, Bratislava, Slovak Republic.
Nahrung. 1995;39(4):257-61. doi: 10.1002/food.19950390402.
We studied the effect of supplementation with vitamins C, E and beta-carotene (PARABION, produced by Syndipharma) on antioxidative status in kidneys of male Wistar rats with diabetes induced by intravenous application of streptozotocin (45 mg.kg-1 of body weight). The animals received subtherapeutic doses of Insulin Interdep (6 U.kg-1 of body weight). A significant decrease of malondialdehyde (MDA), reduced (GSH) and oxidized (GSSG) glutathione and reduction of the activities of Se-glutathione peroxidase (Se-GSH-PX, EC. 1.11.1.9.) and glutathione S-transferase (GST, EC. 2.5.1.18.) were observed in kidneys of diabetic rats treated with these vitamins. On the contrary, the activity of CuZn-superoxide dismutase (CuZn-SOD, EC. 1.15.1.1) and the level of vitamin C (vit. C) increased significantly. No changes were observed for vitamin E (vit. E), beta-carotene and catalase (CAT, EC. 1.11.1.6). Supplementation with vitamins C, E and beta-carotene resulted in an improvement of antioxidative status of kidneys of rats with streptozotocin-induced diabetes.
我们研究了补充维生素C、E和β-胡萝卜素（Syndipharma生产的PARABION）对通过静脉注射链脲佐菌素（45毫克/千克体重）诱导糖尿病的雄性Wistar大鼠肾脏抗氧化状态的影响。这些动物接受了亚治疗剂量的胰岛素（6单位/千克体重）。在接受这些维生素治疗的糖尿病大鼠肾脏中，观察到丙二醛（MDA）、还原型（GSH）和氧化型（GSSG）谷胱甘肽显著减少，以及硒谷胱甘肽过氧化物酶（Se-GSH-PX，EC. 1.11.1.9.）和谷胱甘肽S-转移酶（GST，EC. 2.5.1.18.）的活性降低。相反，铜锌超氧化物歧化酶（CuZn-SOD，EC. 1.15.1.1）的活性和维生素C（vit. C）的水平显著增加。维生素E（vit. E）、β-胡萝卜素和过氧化氢酶（CAT，EC. 1.11.1.6）未观察到变化。补充维生素C、E和β-胡萝卜素可改善链脲佐菌素诱导糖尿病大鼠肾脏的抗氧化状态。
