从腺病毒2型核蛋白的连接位点-Ser(p5'CATCAT)-Gly-Asp-固相合成核肽。汇聚法与逐步合成法策略
Solid-phase synthesis of a nucleopeptide from the linking site of adenovirus-2 nucleoprotein, -Ser(p5'CATCAT)-Gly-Asp-. Convergent versus stepwise strategy.
作者信息
Robles J, Pedroso E, Grandas A
机构信息
Departament de Química Orgànica, Facultat de Química, Universitat de Barcelona, Barcelona, Spain.
出版信息
Nucleic Acids Res. 1995 Oct 25;23(20):4151-61. doi: 10.1093/nar/23.20.4151.
Abstract
The synthesis of a nucleopeptide with the sequence -Ser(p5'CATCAT)-Gly-Asp- has been undertaken by either convergent or stepwise solid-phase strategies, both of which use base-labile permanent protecting groups. The coupling of phosphitylated protected peptides onto oligonucleotide-resins did not afford the desired nucleopeptide, which was nevertheless obtained after oligonucleotide elongation at the hydroxyl group of the resin-bound peptide and deprotection under mild basic conditions. A preliminary study on the stability of different nucleopeptides to bases is also reported.
摘要
已经通过收敛或逐步固相策略进行了序列为-Ser(p5'CATCAT)-Gly-Asp-的核肽的合成，这两种策略都使用对碱不稳定的永久性保护基团。将磷酸化的保护肽偶联到寡核苷酸树脂上并没有得到所需的核肽，不过在树脂结合肽的羟基上进行寡核苷酸延伸并在温和碱性条件下脱保护后得到了该核肽。还报道了对不同核肽对碱稳定性的初步研究。
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