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从腺病毒2型核蛋白的连接位点-Ser(p5'CATCAT)-Gly-Asp-固相合成核肽。汇聚法与逐步合成法策略

Solid-phase synthesis of a nucleopeptide from the linking site of adenovirus-2 nucleoprotein, -Ser(p5'CATCAT)-Gly-Asp-. Convergent versus stepwise strategy.

作者信息

Robles J, Pedroso E, Grandas A

机构信息

Departament de Química Orgànica, Facultat de Química, Universitat de Barcelona, Barcelona, Spain.

出版信息

Nucleic Acids Res. 1995 Oct 25;23(20):4151-61. doi: 10.1093/nar/23.20.4151.

DOI:10.1093/nar/23.20.4151
PMID:7479079
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307357/
Abstract

The synthesis of a nucleopeptide with the sequence -Ser(p5'CATCAT)-Gly-Asp- has been undertaken by either convergent or stepwise solid-phase strategies, both of which use base-labile permanent protecting groups. The coupling of phosphitylated protected peptides onto oligonucleotide-resins did not afford the desired nucleopeptide, which was nevertheless obtained after oligonucleotide elongation at the hydroxyl group of the resin-bound peptide and deprotection under mild basic conditions. A preliminary study on the stability of different nucleopeptides to bases is also reported.

摘要

已经通过收敛或逐步固相策略进行了序列为-Ser(p5'CATCAT)-Gly-Asp-的核肽的合成，这两种策略都使用对碱不稳定的永久性保护基团。将磷酸化的保护肽偶联到寡核苷酸树脂上并没有得到所需的核肽，不过在树脂结合肽的羟基上进行寡核苷酸延伸并在温和碱性条件下脱保护后得到了该核肽。还报道了对不同核肽对碱稳定性的初步研究。

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本文引用的文献

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Studies on the synthesis of nucleotidyl-peptides. II. The preparation of a nucleotidyl-peptide having a 5'-nucleotidyl-(P-O)-serine phosphodiester bond.核苷酸肽的合成研究。II. 具有5'-核苷酸-(P-O)-丝氨酸磷酸二酯键的核苷酸肽的制备。
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Preparation of an aspartic acid-containing protected peptide. Alternatives for overcoming a side reaction during HF treatment.含天冬氨酸的保护肽的制备。克服HF处理过程中副反应的替代方法。
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Pneumococcal bacteriophage Cp-1 contains a protein bound to the 5' termini of its DNA.肺炎球菌噬菌体Cp-1含有一种与DNA 5'末端结合的蛋白质。
Virology. 1983 Jul 15;128(1):92-104. doi: 10.1016/0042-6822(83)90321-5.
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Primary structure, gene organization and polypeptide expression of poliovirus RNA.脊髓灰质炎病毒RNA的一级结构、基因组织及多肽表达
Nature. 1981 Jun 18;291(5816):547-53. doi: 10.1038/291547a0.
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Location of the serine residue involved in the linkage between the terminal protein and the DNA of phage phi 29.
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