脊髓灰质炎病毒核蛋白RNA核肽片段的固相合成。
Solid-phase synthesis of an RNA nucleopeptide fragment from the nucleoprotein of poliovirus.
作者信息
Dreef-Tromp C M, van den Elst H, van den Boogaart J E, van der Marel G A, van Boom J H
机构信息
Gorlaeus Laboratory, Leiden, The Netherlands.
出版信息
Nucleic Acids Res. 1992 May 25;20(10):2435-9. doi: 10.1093/nar/20.10.2435.
Abstract
The naturally occurring RNA-nucleopeptide H-Ala-Tyr[5'-pUUAAAAC-3']-NH2 is prepared via a solid-phase phosphite triester approach using N-SiOMB/O-TBDMS-protected nucleosides. Preliminary 1H-NMR studies show that the peptidyl unit has a remarkable effect on the conformational behaviour of the RNA moiety in the nucleopeptide.
摘要
天然存在的RNA-核肽H-Ala-Tyr[5'-pUUAAAAC-3']-NH2是通过使用N-SiOMB/O-TBDMS保护的核苷的固相亚磷酸三酯方法制备的。初步的1H-NMR研究表明，肽基单元对核肽中RNA部分的构象行为有显著影响。
相似文献
1
Solid-phase synthesis of an RNA nucleopeptide fragment from the nucleoprotein of poliovirus.脊髓灰质炎病毒核蛋白RNA核肽片段的固相合成。
Nucleic Acids Res. 1992 May 25;20(10):2435-9. doi: 10.1093/nar/20.10.2435.
2
Fully automated sequential solid phase approach towards viral RNA-nucleopeptides.全自动顺序固相法制备病毒 RNA-核肽。
Chem Commun (Camb). 2012 Aug 21;48(65):8093-5. doi: 10.1039/c2cc33477a. Epub 2012 Jul 10.
3
Solid-phase synthesis of the nucleopeptide fragment H-Asp-Ser[pAAAGTAAGCC]-Glu-OH from the nucleoprotein of Bacillus subtilis phage phi 29.来自枯草芽孢杆菌噬菌体phi 29核蛋白的核肽片段H-Asp-Ser[pAAAGTAAGCC]-Glu-OH的固相合成
Nucleic Acids Res. 1992 Aug 11;20(15):4015-20. doi: 10.1093/nar/20.15.4015.
4
Chemical synthesis of picornaviral protein primers of RNA replication.
Org Biomol Chem. 2006 Oct 7;4(19):3576-86. doi: 10.1039/b608544j. Epub 2006 Aug 30.
5
Antitermination in bacteriophage lambda. The structure of the N36 peptide-boxB RNA complex.噬菌体λ中的抗终止作用。N36肽-boxB RNA复合物的结构。
Eur J Biochem. 2000 Apr;267(8):2397-408. doi: 10.1046/j.1432-1327.2000.01251.x.
6
The crystal structure and RNA-binding of an orthomyxovirus nucleoprotein.正粘病毒核蛋白的晶体结构与 RNA 结合。
PLoS Pathog. 2013 Sep;9(9):e1003624. doi: 10.1371/journal.ppat.1003624. Epub 2013 Sep 12.
7
[Chemical synthesis of nucleopeptide].[核肽的化学合成]
Tanpakushitsu Kakusan Koso. 1995 Jul;40(10):1342-8.
8
Solid-phase synthesis of H-Phe-Tyr-(pATAT)-NH2: a nucleopeptide fragment from the nucleoprotein of bacteriophage phi X174.H-Phe-Tyr-(pATAT)-NH2的固相合成：来自噬菌体phi X174核蛋白的一个核肽片段。
Nucleic Acids Res. 1990 Nov 25;18(22):6491-5. doi: 10.1093/nar/18.22.6491.
9
10
Structure and assembly of the influenza A virus ribonucleoprotein complex.流感 A 病毒核糖核蛋白复合物的结构与组装。
FEBS Lett. 2013 Apr 17;587(8):1206-14. doi: 10.1016/j.febslet.2013.02.048. Epub 2013 Mar 13.
引用本文的文献
1
Synthesis of Sensitive RNAs Using Fluoride-Cleavable Groups as Linkers and Amino-Group Protection.使用氟化物可裂解基团作为连接子和氨基保护基团合成敏感RNA。
Angew Chem Int Ed Engl. 2025 Jun 17;64(25):e202424560. doi: 10.1002/anie.202424560. Epub 2025 Apr 17.
2
Chemistry of Peptide-Oligonucleotide Conjugates: A Review.肽核酸缀合物的化学：综述。
Molecules. 2021 Sep 6;26(17):5420. doi: 10.3390/molecules26175420.
3
NMR structure of the viral peptide linked to the genome (VPg) of poliovirus.与脊髓灰质炎病毒基因组相连的病毒肽（VPg）的核磁共振结构。
Peptides. 2006 Jul;27(7):1676-84. doi: 10.1016/j.peptides.2006.01.018. Epub 2006 Mar 15.
4
Biochemical and genetic studies of the VPg uridylylation reaction catalyzed by the RNA polymerase of poliovirus.脊髓灰质炎病毒RNA聚合酶催化的VPg尿苷酸化反应的生化及遗传学研究
J Virol. 2003 Jan;77(2):891-904. doi: 10.1128/jvi.77.2.891-904.2003.
5
Identification of an RNA hairpin in poliovirus RNA that serves as the primary template in the in vitro uridylylation of VPg.鉴定脊髓灰质炎病毒RNA中的一个RNA发夹结构，该结构在体外VPg尿苷酸化过程中作为主要模板。
J Virol. 2000 Nov;74(22):10359-70. doi: 10.1128/jvi.74.22.10359-10370.2000.
6
Solid-phase synthesis of a nucleopeptide from the linking site of adenovirus-2 nucleoprotein, -Ser(p5'CATCAT)-Gly-Asp-. Convergent versus stepwise strategy.从腺病毒2型核蛋白的连接位点-Ser(p5'CATCAT)-Gly-Asp-固相合成核肽。汇聚法与逐步合成法策略
Nucleic Acids Res. 1995 Oct 25;23(20):4151-61. doi: 10.1093/nar/23.20.4151.
本文引用的文献
1
[FORMS OF CHEMICAL BONDS BETWEEN RNA, PROTEINS AND THEIR FRACTIONS].[RNA、蛋白质及其组分之间的化学键形式]
Usp Sovrem Biol. 1963 May-Jun;55:321-38.
2
Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.寡脱氧核糖核苷酸的快速合成。VII. 在硅藻土-聚酰胺载体上通过连续流动磷酸三酯法固相合成寡脱氧核糖核苷酸。
Nucleic Acids Res. 1982 Oct 25;10(20):6243-54. doi: 10.1093/nar/10.20.6243.
3
Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.聚合物载体寡核苷酸合成 XVIII：脱氧核苷的β-氰基乙基-N，N-二烷基氨基-/N-吗啉代亚磷酰胺用于 DNA 片段合成，简化最终产物的脱保护和分离
Nucleic Acids Res. 1984 Jun 11;12(11):4539-57. doi: 10.1093/nar/12.11.4539.
4
Synthetic nucleotide-peptides.合成核苷酸肽
Prog Nucleic Acid Res Mol Biol. 1970;10:145-82. doi: 10.1016/s0079-6603(08)60564-4.
5
Proteins covalently linked to viral genomes.与病毒基因组共价连接的蛋白质。
Prog Nucleic Acid Res Mol Biol. 1987;34:209-51. doi: 10.1016/s0079-6603(08)60497-3.
6
Conformational analysis of the tetranucleotides m6(2)A-m6(2)A-U-m6(2)A(m6(2)A = N6-dimethyladenosine) and U-m6(2)A-U-m6(2)A and of the hybrid dA-r(U-A). A one- and two-dimensional NMR study.四核苷酸m6(2)A-m6(2)A-U-m6(2)A（m6(2)A = N6-二甲基腺苷）、U-m6(2)A-U-m6(2)A以及杂交体dA-r(U-A)的构象分析。一维和二维核磁共振研究。
Eur J Biochem. 1988 Jan 15;171(1-2):155-62. doi: 10.1111/j.1432-1033.1988.tb13771.x.
7
A model study directed towards the preparation of nucleopeptides via H-phosphonate intermediates.一项旨在通过H-膦酸酯中间体制备核肽的模型研究。
Nucleic Acids Res. 1987 Feb 25;15(4):1807-18. doi: 10.1093/nar/15.4.1807.
8
Synthesis of the nucleopeptides H-Phe-Tyr(pGC)-NH2 and H-Phe-Ser(pGC)-Ala-OH via a phosphotriester approach.通过磷酸三酯法合成核肽H-Phe-Tyr(pGC)-NH2和H-Phe-Ser(pGC)-Ala-OH。
Nucleic Acids Res. 1989 Apr 25;17(8):2897-905. doi: 10.1093/nar/17.8.2897.
9
Solid-phase synthesis of H-Phe-Tyr-(pATAT)-NH2: a nucleopeptide fragment from the nucleoprotein of bacteriophage phi X174.H-Phe-Tyr-(pATAT)-NH2的固相合成：来自噬菌体phi X174核蛋白的一个核肽片段。
Nucleic Acids Res. 1990 Nov 25;18(22):6491-5. doi: 10.1093/nar/18.22.6491.
10
Facile thiolytic removal of the o-nitrophenylsulphenyl amino-protecting group.邻硝基苯硫基氨基保护基团的简便硫解去除法。
Int J Pept Protein Res. 1979 Mar;13(3):315-9. doi: 10.1111/j.1399-3011.1979.tb01885.x.
Zotero 插件