Ahmed M, Srinivasan G R, Theodorsson E, Schultzberg M, Kreicbergs A
Department of Orthopaedics, Karolinska Hospital, Stockholm, Sweden.
Peptides. 1995;16(4):569-79. doi: 10.1016/0196-9781(95)00025-f.
The occurrence of substance P (SP) and calcitonin gene-related peptide (CGRP) was assessed in the ankle joints and dorsal root ganglia (L2-L6) by immunohistochemistry and radioimmunoassay (RIA) after unilateral sciatic nerve section in adjuvant arthritis. Nerve section in arthritic rats had no clear-cut effect on warmth, redness, and swelling of ankle joints, but significantly affected the occurrence of SP and CGRP. Immunohistochemical analysis showed an almost complete disappearance of SP-positive fibers in the ipsilateral ankle joint in normal rats after axotomy, whereas in arthritic rats occasional SP-positive fibers remained. In dorsal root ganglia, only occasional SP-positive cells could be detected in normal and arthritic rats after axotomy. A similar but somewhat less pronounced effect of axotomy was noted for neuronal CGRP-LI. RIA showed a decrease in SP in ankle joints by 45% in normal rats and 58% in arthritic rats; the decrease in CGRP was 41% and 47%, respectively. In dorsal root ganglia, the decrease in SP after surgical denervation was 25% in normal rats and 54% in arthritic rats; the decrease in CGRP was 18% and 27%, respectively. The tissue concentrations of SP and CGRP in ipsilateral ankle joints and dorsal root ganglia were consistently correlated in normal as well as arthritic rats. The present study shows that an interruption of the nerve supply to joints cannot fully prevent the development of arthritis, although it significantly reduces the occurrence of sensory neuropeptides.
采用免疫组织化学和放射免疫分析法（RIA），在佐剂性关节炎大鼠单侧坐骨神经切断术后，评估踝关节和背根神经节（L2-L6）中P物质（SP）和降钙素基因相关肽（CGRP）的表达情况。切断关节炎大鼠的神经对踝关节的发热、发红和肿胀没有明显影响，但对SP和CGRP的表达有显著影响。免疫组织化学分析显示，正常大鼠坐骨神经切断术后，同侧踝关节SP阳性纤维几乎完全消失，而在关节炎大鼠中仍有偶尔的SP阳性纤维残留。在背根神经节中，切断神经后，正常和关节炎大鼠中仅偶尔能检测到SP阳性细胞。对于神经元CGRP-LI，切断神经也有类似但稍弱的影响。RIA显示，正常大鼠踝关节中SP含量降低45%，关节炎大鼠降低58%；CGRP的降低分别为41%和47%。在背根神经节中，手术去神经后，正常大鼠SP降低25%，关节炎大鼠降低54%；CGRP的降低分别为18%和27%。在正常和关节炎大鼠中，同侧踝关节和背根神经节中SP和CGRP的组织浓度始终相关。本研究表明，尽管神经供应中断会显著减少感觉神经肽的表达，但不能完全阻止关节炎的发展。
