Di Marcotullio L, Buttinelli M, Costanzo G, Di Mauro E, Negri R
Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, P. le Aldo Moro, 5 00185 Roma, Italy.
Biochem J. 1998 Jul 1;333 ( Pt 1)(Pt 1):65-9. doi: 10.1042/bj3330065.
The effects of the rotational information of DNA in determining the in vivo localization of nucleosomal core particles (ncps) have been studied in the Saccharomyces cerevisiae 5 S rRNA repeat gene. The distribution of the phased series of flexibility signals present in this DNA has been altered by inserting in its centre a 25 bp tract. The effects of such alteration on the in vivo distribution of the helically phased, alternatively located ncps have been determined relative to a reference 21 bp insertion mutant. The results show that the answers provided in vitro and in vivo by the yeast 5 S rRNA gene sequence to specific modifications of the DNA rotational frame are similar, thus pointing to the relevance of DNA rotational information in vivo.
在酿酒酵母5S rRNA重复基因中，研究了DNA的旋转信息在确定核小体核心颗粒（ncps）体内定位方面的作用。通过在该DNA中心插入一段25bp的序列，改变了此DNA中存在的相位系列灵活性信号的分布。相对于一个参考的21bp插入突变体，已确定了这种改变对螺旋相位交替定位的ncps体内分布的影响。结果表明，酵母5S rRNA基因序列在体外和体内对DNA旋转框架特定修饰所给出的答案相似，从而表明DNA旋转信息在体内具有相关性。
