Waterston R, Sulston J
Department of Genetics and Genome Sequencing Center, Washington University School of Medicine, St. Louis, MO 63110, USA.
Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):10836-40. doi: 10.1073/pnas.92.24.10836.
The physical map of the 100-Mb Caenorhabditis elegans genome consists of 17,500 cosmids and 3500 yeast artificial chromosomes (YACs). A total of 22.5 Mb has been sequenced, with the remainder expected by 1998. A further 15.5 Mb of unfinished sequence is freely available online: because the areas sequenced so far are relatively gene rich, about half the 13,000 genes can now be scanned. More than a quarter of the genes are represented by expressed sequence tags (ESTs). All information pertaining to the genome is publicly available in the ACeDB data base.
100兆碱基的秀丽隐杆线虫基因组的物理图谱由17500个黏粒和3500个酵母人工染色体(YAC)组成。总共22.5兆碱基已被测序,其余部分预计在1998年完成。另外15.5兆碱基的未完成序列可在网上免费获取:由于目前已测序的区域基因相对丰富,现在可以扫描约13000个基因中的一半。超过四分之一的基因由表达序列标签(EST)代表。所有与基因组相关的信息都可在ACeDB数据库中公开获取。