由酵母人工染色体（YAC）DNA制备的M13文库的构建与分析。
The construction and analysis of M13 libraries prepared from YAC DNA.
作者信息
Vaudin M, Roopra A, Hillier L, Brinkman R, Sulston J, Wilson R K, Waterston R H
机构信息
Department of Genetics, Washington University School of Medicine, St. Louis, MO 63108.
出版信息
Nucleic Acids Res. 1995 Feb 25;23(4):670-4. doi: 10.1093/nar/23.4.670.
Yeast artificial chromosomes (YACs) provide a powerful way to isolate and map large regions of genomic DNA and their use in genome analysis is now extensive. We modified a series of procedures to produce high quality shotgun libraries from small amounts of YAC DNA. Clones from several different libraries have been sequenced and analyzed for distribution, sequence integrity and degree of contamination from yeast DNA. We describe these procedures and analyses and show that sequencing at about 1-fold coverage, followed by database comparison (survey sequencing) offers a relatively quick method to determine the nature of previously uncharacterized cosmid or YAC clones.
酵母人工染色体（YACs）为分离和绘制基因组DNA大片段提供了一种强大的方法，其在基因组分析中的应用现已十分广泛。我们改进了一系列程序，以便从少量YAC DNA中构建高质量的鸟枪法文库。对来自几个不同文库的克隆进行了测序，并分析了它们的分布、序列完整性以及酵母DNA的污染程度。我们描述了这些程序和分析方法，并表明以约1倍覆盖率进行测序，随后进行数据库比较（普查测序），提供了一种相对快速的方法来确定以前未表征的黏粒或YAC克隆的性质。
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