Oudkerk Pool M, Bouma G, Visser J J, Kolkman J J, Tran D D, Meuwissen S G, Peña A S
Dept. of Gastroenterology, Free University Hospital, Amsterdam, The Netherlands.
Scand J Gastroenterol. 1995 Aug;30(8):784-8. doi: 10.3109/00365529509096328.
Nitric oxide is an important mediator in inflammatory and autoimmune-mediated tissue destruction and may be of pathophysiologic importance in inflammatory bowel disease. We studied whether serum levels of nitrate, the stable end-product of nitric oxide, are increased in active Crohn's disease or ulcerative colitis, in comparison with quiescent disease and healthy controls. The setting was the gastroenterology unit of the Free University Hospital, Amsterdam.
In 146 patients--75 with ulcerative colitis and 71 with Crohn's disease--and 33 controls serum nitrate was measured by the Griess reaction after enzymatic conversion of nitrate to nitrite with nitrate reductase.
Median serum nitrate concentrations did not differ statistically significantly between ulcerative colitis (median, 34.2 mumol/l; range, 15.6-229.4 mumol/l), Crohn's disease (median 32.3 mumol; range 13.2-143.2 mumol/l), and healthy controls (median, 28.7 mumol/l; range, 13.0-108.4 mumol/l). However, when active ulcerative colitis patients (median, 44 mumol/l; range, 29.1-229.4 mumol/l were compared with inactive ulcerative colitis patients (median, 31.2 mumol/l; range, 15.6-59.7 mumol/l), a significant difference in nitrate concentration was found (p < 0.0001). A significant positive correlation was found between serum nitrate levels in ulcerative colitis and erythrocyte sedimentation rate (ESR) (r = 0.30, p - 0.01), leucocyte count (r = 0.27, p = 0.02), and thrombocyte count (r = 0.24, p = 0.04). Comparing active Crohn's disease patients (median, 37.5 mumol/l; range, 13.2-143.2 mumol/l) with inactive Crohn's disease patients (median, 31.3 mumol/l; range, 14.5-92.3 mumol/l) also showed a significant difference in serum nitrate concentration (p < 0.009). Serum nitrate levels correlated with the ESR (r = 0.26, p = 0.028) and serum albumin (r = 0.38, p = 0.004) as well.
Nitric oxide production is increased in both active ulcerative colitis and Crohn's disease and may be implicated in the pathogenesis of inflammatory bowel disease.
一氧化氮是炎症和自身免疫介导的组织破坏中的重要介质，在炎症性肠病中可能具有病理生理学意义。我们研究了与静止期疾病和健康对照相比，一氧化氮的稳定终产物硝酸盐的血清水平在活动性克罗恩病或溃疡性结肠炎中是否升高。研究地点为阿姆斯特丹自由大学医院的胃肠病科。
对146例患者（75例溃疡性结肠炎患者和71例克罗恩病患者）及33名对照者，用硝酸还原酶将硝酸盐酶促转化为亚硝酸盐后，通过格里斯反应测定血清硝酸盐。
溃疡性结肠炎（中位数34.2μmol/L；范围15.6 - 229.4μmol/L）、克罗恩病（中位数32.3μmol；范围13.2 - 143.2μmol/L）和健康对照者（中位数28.7μmol/L；范围13.0 - 108.4μmol/L）的血清硝酸盐浓度中位数在统计学上无显著差异。然而，当将活动性溃疡性结肠炎患者（中位数44μmol/L；范围29.1 - 229.4μmol/L）与非活动性溃疡性结肠炎患者（中位数31.2μmol/L；范围15.6 - 59.7μmol/L）进行比较时，发现硝酸盐浓度有显著差异（p < 0.0001）。在溃疡性结肠炎中，血清硝酸盐水平与红细胞沉降率（ESR）（r = 0.30，p = 0.01）、白细胞计数（r = 0.27，p = 0.02）和血小板计数（r = 0.24，p = 0.04）之间存在显著正相关。将活动性克罗恩病患者（中位数37.5μmol/L；范围13.2 - 143.2μmol/L）与非活动性克罗恩病患者（中位数31.3μmol/L；范围14.5 - 92.3μmol/L）进行比较，也显示出血清硝酸盐浓度有显著差异（p < 0.009）。血清硝酸盐水平还与ESR（r = 0.26，p = 0.028）和血清白蛋白（r = 0.38，p = 0.004）相关。
活动性溃疡性结肠炎和克罗恩病中一氧化氮的产生均增加，可能与炎症性肠病的发病机制有关。
