Bağdaş Ancel Aysun, Barutçu Sezgin, Saracaloğlu Ahmet, Demiryürek Abdullah Tuncay
Department of Gastroenterology,Gaziantep University Faculty of Medicine, Gaziantep, Türkiye.
Department of Medical Pharmacology, Gaziantep University Faculty of Medicine, Gaziantep, Türkiye.
Turk J Gastroenterol. 2024 Nov 25;36(2):114-123. doi: 10.5152/tjg.2024.23519.
BACKGROUND/AIMS: Crohn's disease (CD) is a major subtype of chronic relapsing inflammatory gastrointestinal disorders. In this study, we assessed the possible contributions of serum oxidative/nitrosative stress and dynamic thiol/disulfide homeostasis to CD pathogenesis.
Patients with active CD (A-CD) at onset (n = 38), CD patients in the remission (R-CD) (n = 38), and healthy controls (n = 38) were prospectively included in this study. Serum oxidative/nitrosative parameters as well as total thiol and native thiol levels were analyzed.
We observed significant augmentation in nitric oxide (NO) levels in both A-CD and R-CD patients compared to healthy controls. We detected marked reductions in the 3-nitrotyrosine levels in the patient groups. Glutathione, glutathione peroxidase, and myeloperoxidase levels were observed to be significantly lower in both the active and remission groups (P < .001). In the A-CD group, native thiol (P < .001) and total thiol (P < .01) levels were lower, and disulfide levels were higher than those of the control group (P < .01), while the native thiol/total thiol ratio was reduced and disulfide/total thiol (P < .001) and disulfide/native thiol (P < .001) ratios were elevated. Remarkably, no change in dynamic thiol/disulfide homeostasis was found in the R-CD group.
Our results showed increased serum NO levels and decreased antioxidant enzymes, particularly during the active phase of CD. Determination of thiol/disulfide homeostasis could help differentiate between the active and remission phases of the disease. Thiol/ disulfide parameters can be used as biomarkers for A-CD.
背景/目的：克罗恩病（CD）是慢性复发性炎症性胃肠疾病的一种主要亚型。在本研究中，我们评估了血清氧化/亚硝化应激以及动态硫醇/二硫键稳态对CD发病机制的可能作用。
本研究前瞻性纳入了38例初发活动期CD（A-CD）患者、38例缓解期CD（R-CD）患者以及38例健康对照者。分析了血清氧化/亚硝化参数以及总硫醇和天然硫醇水平。
与健康对照相比，我们观察到A-CD和R-CD患者的一氧化氮（NO）水平均显著升高。我们检测到患者组中3-硝基酪氨酸水平显著降低。活性组和缓解组的谷胱甘肽、谷胱甘肽过氧化物酶和髓过氧化物酶水平均显著降低（P <.001）。在A-CD组中，天然硫醇水平（P <.001）和总硫醇水平（P <.01）较低，二硫键水平高于对照组（P <.01），而天然硫醇/总硫醇比值降低，二硫键/总硫醇（P <.001）和二硫键/天然硫醇（P <.001）比值升高。值得注意的是，R-CD组的动态硫醇/二硫键稳态未发现变化。
我们的结果显示血清NO水平升高，抗氧化酶减少，尤其是在CD的活动期。硫醇/二硫键稳态的测定有助于区分疾病的活动期和缓解期。硫醇/二硫键参数可作为A-CD的生物标志物。
