乳胶凝集试验、对流免疫电泳及葡萄球菌A蛋白协同凝集试验在细菌性脑膜炎诊断中的应用
Latex agglutination, counterimmunoelectrophoresis, and protein A co-agglutination in diagnosis of bacterial meningitis.
作者信息
Dirks-Go S I, Zanen H C
出版信息
J Clin Pathol. 1978 Dec;31(12):1167-71. doi: 10.1136/jcp.31.12.1167.
Abstract
Specimens of cerebrospinal fluid from 201 patients with meningitis caused by Neisseria meningitidis groups A/B/C/135, Streptococcus pneumoniae (23 types), and Haemophilus influenzae type b were tested for the presence of specific bacterial antigens by latex agglutination, counterimmunoelectrophoresis (CIE), and protein A co-agglutination. Specific antigens were found in 75% of the specimens by latex agglutination and CIE, and in 60% of the specimens by protein A co-agglutination. Non-specific reactions in protein A co-agglutination were prevented by heating the specimens to 100 degrees C for a few minutes. The three methods are simple and quick to perform. The smallest amount of antiserum was used in protein A co-agglutination, but we found this method less sensitive. Latex agglutination and CIE proved to be equally sensitive, but the first method was easier to adopt in practice.
摘要
对201例由A/B/C/135群脑膜炎奈瑟菌、肺炎链球菌（23种类型）和b型流感嗜血杆菌引起的脑膜炎患者的脑脊液标本进行检测，采用乳胶凝集试验、对流免疫电泳（CIE）和蛋白A协同凝集试验检测特定细菌抗原的存在情况。通过乳胶凝集试验和CIE在75%的标本中发现了特定抗原，通过蛋白A协同凝集试验在60%的标本中发现了特定抗原。通过将标本加热至100摄氏度几分钟可防止蛋白A协同凝集试验中的非特异性反应。这三种方法操作简单快捷。蛋白A协同凝集试验使用的抗血清量最少，但我们发现该方法不太敏感。乳胶凝集试验和CIE证明同样敏感，但第一种方法在实际应用中更容易采用。
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