Murphree R L, Tamplin M L
Department of Biology, Jacksonville State University, Alabama 36265, USA.
Appl Environ Microbiol. 1995 Oct;61(10):3656-60. doi: 10.1128/aem.61.10.3656-3660.1995.
Vibrio cholerae 01, the causative agent of cholera, is known to persist in estuarine environments as endogenous microflora. The recent introduction of V. cholerae 01 into estuaries of the North and South American continents has stimulated the need to determine the effect of controlled purification on reducing this pathogen in edible molluscan shellfish. Experiments defined parameters for the uptake and retention of V. cholerae 01 in tissues of Crassostrea virginica, and these parameters were compared with those for Escherichia coli and Salmonella tallahassee, bacteria which are usually eliminated from moderately contaminated shellfish within 48 h. Oysters accumulated greater concentrations of V. cholerae 01 than E. coli and S. tallahassee. When V. cholerae 01 was exposed to controlled purification at 15, 19 and 25 degrees C over 48 h, it persisted in oysters at markedly higher levels than E. coli and S. tallahassee. The concentration of a V. cholerae 01-specific agglutinin did not positively correlate with the uptake or retention of V. cholerae 01. These data show that state and federally approved controlled purification techniques are not effective at reducing V. cholerae 01 in oysters.
霍乱弧菌01群是霍乱的病原体，已知它作为内源性微生物群存在于河口环境中。最近霍乱弧菌01群传入南北美洲大陆的河口，促使人们有必要确定控制净化对减少可食用软体贝类中这种病原体的效果。实验确定了弗吉尼亚海湾扇贝组织摄取和留存霍乱弧菌01群的参数，并将这些参数与大肠杆菌和塔拉哈西沙门氏菌的参数进行比较，后两种细菌通常在48小时内从中度污染的贝类中被清除。牡蛎积累的霍乱弧菌01群浓度高于大肠杆菌和塔拉哈西沙门氏菌。当霍乱弧菌01群在15、19和25摄氏度下接受48小时的控制净化时，它在牡蛎中的留存水平明显高于大肠杆菌和塔拉哈西沙门氏菌。霍乱弧菌01群特异性凝集素的浓度与霍乱弧菌01群的摄取或留存没有正相关关系。这些数据表明，州和联邦政府批准的控制净化技术在减少牡蛎中霍乱弧菌01群方面无效。
