Ficorilli N, Studdert M J, Crabb B S
Centre for Equine Virology, School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Arch Virol. 1995;140(9):1653-62. doi: 10.1007/BF01322539.
The nucleotide sequence of the glycoprotein G (gG) homologue of asinine herpesvirus 3 (AHV3), a respiratory alphaherpesvirus of donkeys, was determined. The AHV3 gG gene consists of 1233 base pairs (bp) and codes for a predicted protein of 411 amino acids. This is identical in size to the equine herpesvirus 1 (EHV1) gG gene and 6 amino acids longer than the equine herpesvirus 4 (EHV4) gG gene. The predicted amino acid sequence of AHV3 gG has characteristics of a class 1 membrane protein. The amino acid sequence of AHV3 gG shows 92% and 60% identity to EHV1 gG and EHV4 gG respectively. Two regions within the gG amino acid sequences of EHV1 and EHV4 were previously defined, an N-terminal constant region and an immunodominant highly variable region located toward the C-terminus. In the corresponding constant region of AHV3 gG there was 96% and 75% amino acid identity with EHV1 and EHV4 gGs respectively. In the variable region, there was 73% and 24% identity respectively. Phylogenetic analyses using the gG nucleotide sequences indicated that AHV3 is much closer in evolutionary distance to EHV1 than either virus is to EHV4. These findings provide additional support for the view that AHV3, or another closely related virus, may be the progenitor of EHV1 and has adapted to horses in relatively recent times.
测定了驴的呼吸道甲型疱疹病毒——驴疱疹病毒3（AHV3）糖蛋白G（gG）同源物的核苷酸序列。AHV3 gG基因由1233个碱基对（bp）组成，编码一个预测的含411个氨基酸的蛋白质。其大小与马疱疹病毒1（EHV1）的gG基因相同，比马疱疹病毒4（EHV4）的gG基因长6个氨基酸。预测的AHV3 gG氨基酸序列具有1类膜蛋白的特征。AHV3 gG的氨基酸序列与EHV1 gG和EHV4 gG的同一性分别为92%和60%。EHV1和EHV4的gG氨基酸序列中先前定义了两个区域，一个N端恒定区和一个位于C端的免疫显性高变区。在AHV3 gG的相应恒定区，与EHV1和EHV4 gG的氨基酸同一性分别为96%和75%。在可变区，同一性分别为73%和24%。使用gG核苷酸序列进行的系统发育分析表明，AHV3在进化距离上与EHV1比与EHV4更接近。这些发现为AHV3或另一种密切相关病毒可能是EHV1的祖先且在相对较近的时期适应了马这一观点提供了额外支持。
