Chaiyaroj S C, Sirisereewan T, Jiamwatanasuk N, Sirisinha S
Department of Microbiology, Faculty of Science, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Asian Pac J Allergy Immunol. 1995 Jun;13(1):55-61.
A monoclonal antibody (MAb 3G6) specific for Campylobacter jejuni and Campylobacter coli was produced by immunizing BALB/c mice with a local strain of C.jejuni (28.1). No cross-reactivity was observed with Enterobacteriaceae controls. By immunoprecipitation, MAb 3G6 identified a major protein band of molecular weight 45 kDa and also gave a slight reactivity with 30 and 55 kDa proteins. Using an indirect enzyme-linked immunosorbent assay, MAb 3G6 was able to detect C.jejuni suspended in stool without cross-reactivity to 14 other enteropathogenic bacteria suspended, normal flora in fecal suspension, or fecal debris. In the analysis of fifty clinical specimens, MAb 3G6 detected most positive samples with the exception of one which possessed very low Campylobacter concentration and gave no reactivity to negative samples, demonstrating its high specificity to C. jejuni and C. coli. MAb 3G6 may be suitable as a new tool for the development of diagnostic method for Campylobacter infection.
用空肠弯曲菌的一株本地菌株（28.1）免疫BALB/c小鼠，制备了一种对空肠弯曲菌和结肠弯曲菌具有特异性的单克隆抗体（MAb 3G6）。未观察到与肠杆菌科对照有交叉反应。通过免疫沉淀，MAb 3G6鉴定出一条分子量为45 kDa的主要蛋白带，并且与30 kDa和55 kDa的蛋白也有轻微反应。使用间接酶联免疫吸附测定法，MAb 3G6能够检测粪便中悬浮的空肠弯曲菌，而与其他14种悬浮的肠道致病菌、粪便悬液中的正常菌群或粪便残渣无交叉反应。在对50份临床标本的分析中，MAb 3G6检测到了大多数阳性样本，但有一份样本中空肠弯曲菌浓度极低且对阴性样本无反应，这表明其对空肠弯曲菌和结肠弯曲菌具有高度特异性。MAb 3G6可能适合作为开发弯曲菌感染诊断方法的一种新工具。
