正常扁桃体和B细胞淋巴瘤中T细胞增殖的免疫组织化学分析。
Immunohistochemical analysis of T cell proliferation in normal tonsil and B cell lymphoma.
作者信息
Wang J, Isaacson P G, Spencer J
机构信息
Department of Histopathology, University College of London Medical School.
出版信息
J Clin Pathol. 1995 Sep;48(9):873-5. doi: 10.1136/jcp.48.9.873.
A double immunohistochemical technique, incorporating MIB1 and CD3, was used to identify proliferating T cells in paraffin wax sections of normal tonsil and B cell lymphomas. The number of double stained T cells as a percentage of the total T cells was then determined. In normal tonsil and follicular lymphoma the follicle centre and T cell zones were counted independently. In normal tonsil very few T cells in the follicle centre expressed MIB1. Proliferating T cells were concentrated in the T cell zones. The same pattern was observed in follicular lymphoma. In contrast, the percentage of T cells expressing MIB1 was higher in mucosa associated lymphoid tissue type lymphoma and lymphocytic lymphoma, suggesting that T cell activation occurs in these tumours. The highest percentage of MIB1 positive T cells was observed in high grade lymphoma. This suggests that transformation to high grade lymphoma is associated with an increase in T cell activation.
采用结合MIB1和CD3的双重免疫组织化学技术，鉴定正常扁桃体和B细胞淋巴瘤石蜡切片中的增殖性T细胞。然后确定双重染色的T细胞数量占总T细胞的百分比。在正常扁桃体和滤泡性淋巴瘤中，分别对滤泡中心和T细胞区进行计数。在正常扁桃体中，滤泡中心极少有T细胞表达MIB1。增殖性T细胞集中在T细胞区。在滤泡性淋巴瘤中观察到相同的模式。相比之下，黏膜相关淋巴组织型淋巴瘤和淋巴细胞性淋巴瘤中表达MIB1的T细胞百分比更高，这表明这些肿瘤中发生了T细胞活化。在高级别淋巴瘤中观察到MIB1阳性T细胞的百分比最高。这表明向高级别淋巴瘤的转化与T细胞活化增加有关。
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