Brytting M, Mousavi-Jazi M, Boström L, Larsson M, Lunderberg J, Ljungman P, Ringdén O, Sundqvist V A
Department of Virology, Swedish Institute for Infectious Disease Control, Stockholm, Sweden.
Transplantation. 1995 Nov 15;60(9):961-5.
Granulocytes, monocytes, and T- and B-lymphocytes were separated from 28 blood samples collected from 5 bone marrow transplant (BMT) recipients. About 40% of granulocyte, monocyte, and B-lymphocyte samples were CMV DNA-positive by polymerase chain reaction in recipients with cytomegalovirus (CMV) infection. CMV DNA was rarely detected in separated T-lymphocytes. Within each of the simultaneously separated paired samples, there were several with single positive cell subtypes. Monocytes, granulocytes, and B-lymphocytes were the single positive samples in some instances. Thus, it is important to have all of the different cell subtypes present in samples for detection of CMV DNA in peripheral blood. We also studied the appearance of CMV DNA in plasma and peripheral blood leukocytes (PBLs) from 351 blood samples collected from 30 BMT recipients during a follow-up period of at least 3 months after BMT. All cell subtypes were represented in the PBL samples. In the 13 recipients who developed symptoms possibly associated with CMV infection or CMV disease, a correlation with the detection of CMV DNA in < or = 2 x 10(5) PBLs was found. In PBLs from 11 of the 13 BMT recipients, CMV DNA was detected before the onset of symptoms. CMV DNA was not detected in < or = 2 x 10(5) PBLs from recipients without CMV infection. The virus load in PBLs decreased during ganciclovir treatment. Nine of the 13 recipients displayed PCR-positive plasma samples, and CMV DNA was detected frequently after the onset of symptoms.
从5名骨髓移植（BMT）受者采集的28份血样中分离出粒细胞、单核细胞、T淋巴细胞和B淋巴细胞。在患有巨细胞病毒（CMV）感染的受者中，约40%的粒细胞、单核细胞和B淋巴细胞样本通过聚合酶链反应检测为CMV DNA阳性。在分离出的T淋巴细胞中很少检测到CMV DNA。在同时分离的每对样本中，有几个样本存在单一阳性细胞亚型。在某些情况下，单核细胞、粒细胞和B淋巴细胞为单一阳性样本。因此，为了检测外周血中的CMV DNA，样本中存在所有不同的细胞亚型很重要。我们还研究了在BMT后至少3个月的随访期内，从30名BMT受者采集的351份血样中血浆和外周血白细胞（PBL）中CMV DNA的出现情况。PBL样本中包含所有细胞亚型。在13名出现可能与CMV感染或CMV疾病相关症状的受者中，发现与在≤2×10⁵个PBL中检测到CMV DNA存在相关性。在13名BMT受者中的11名的PBL中，在症状出现前检测到CMV DNA。在没有CMV感染的受者的≤2×10⁵个PBL中未检测到CMV DNA。在更昔洛韦治疗期间，PBL中的病毒载量下降。13名受者中有9名的血浆样本PCR呈阳性，并且在症状出现后经常检测到CMV DNA。
