来自薤白的甾体皂苷及其对环磷酸腺苷磷酸二酯酶和钠/钾ATP酶的抑制活性。

Steroidal saponins from Allium chinense and their inhibitory activities on cyclic AMP phosphodiesterase and Na+/K+ ATPase.

作者信息

Kuroda M, Mimaki Y, Kameyama A, Sashida Y, Nikaido T

机构信息

School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.

出版信息

Phytochemistry. 1995 Nov;40(4):1071-6. doi: 10.1016/0031-9422(95)00423-5.

DOI:10.1016/0031-9422(95)00423-5

PMID:7492369

Abstract

The saponin fraction prepared from the methanolic extract of Allium chinense bulbs exhibited inhibitory activities on cyclic AMP phosphodiesterase (cAMP PDE) (43.5%) and Na+/K+ ATPase (59.3%) at a sample concentration of 100 micrograms ml-1, respectively. Attempted purification of the active fraction through column chromatography on silica gel and ODS silica gel resulted in the isolation of six steroidal saponins, one of which appeared to be a new compound and one to be the first isolation from a natural source. (25R,S)-5 alpha-Spirostan-3 beta-ol tetrasaccharide showed inhibitory activities on both cAMP PDE and Na+/K+ ATPase, while (25R)-3 beta-hydroxy-5 alpha-spirostan-6-one di- and tri-saccharides inhibited only cAMP PDE.

摘要

从韭菜鳞茎甲醇提取物中制备的皂苷组分，在样品浓度为100微克/毫升时，分别对环磷酸腺苷磷酸二酯酶（cAMP PDE）（43.5%）和钠钾ATP酶（59.3%）表现出抑制活性。尝试通过硅胶柱色谱和ODS硅胶对活性组分进行纯化，结果分离出六种甾体皂苷，其中一种似乎是新化合物，另一种是首次从天然来源分离得到。（25R，S）-5α-螺甾烷-3β-醇四糖对cAMP PDE和钠钾ATP酶均表现出抑制活性，而（25R）-3β-羟基-5α-螺甾烷-6-酮二糖和三糖仅抑制cAMP PDE。